Label-free electrochemical immunosensors (EIs) are simple and sensitive in cancer diagnosis. Electrode materials which can offer a large specific area and excellent electrical conductivity play a critical role in the fabrication of EIs. In our study, ce-MoS₂/AgNR composite was synthesized via Van der Waals force and electrostatic interaction between chemical exfoliated MoS₂ nanosheets (ce-MoS₂) and Ag nanorods (AgNRs). The conductivity of ce-MoS₂/AgNR composite was almost three times higher than single ce-MoS₂ because of the synergistic effect between ce-MoS₂ and AgNRs. Furthermore, AgNRs could effectively inhibit the stacking of MoS₂ nanosheets. The EIs for prostate-specific antigen (PSA) detection were fabricated based on ce-MoS₂/AgNR composite. The fabricated EIs retained a wide linear response range (0.1–1000 ng/ml), low sensitivity (0.019 μA/(ng ml⁻¹)), and low detection limit (0.051 ng/ml) for the detection of PSA. Therefore, the EIs based on screen-printed electrode (SPE) and ce-MoS₂/AgNR composite could be applied to clinical diagnosis of prostatic cancer and have potential application in the detection of other cancer biomarkers.

无标记电化学免疫传感器（EIs）在癌症诊断中既简单又敏感。可以提供大比表面积和出色电导率的电极材料在EI的制造中起着至关重要的作用。在我们的研究中，通过范德华力和化学剥离MoS ₂纳米片（ce-MoS ₂）与Ag纳米棒（AgNRs）之间的静电相互作用合成了ce-MoS ₂ / AgNR复合材料。ce-MoS ₂ / AgNRs复合材料的电导率几乎是单一ce-MoS _2的三倍，因为ce-MoS ₂和AgNRs具有协同作用。此外，AgNRs可以有效抑制MoS ₂的堆积纳米片。基于ce-MoS ₂ / AgNR复合物制备了用于前列腺特异性抗原（PSA）检测的EI。制成的EI保留了宽的线性响应范围（0.1–1000 ng / ml），低灵敏度（0.019μA/（ng ml ^-1））和低检测限（0.051 ng / ml）用于PSA的检测。因此，基于丝网印刷电极（SPE）和ce-MoS ₂ / AgNR复合材料的EIs可以用于前列腺癌的临床诊断，并在检测其他癌症生物标志物方面具有潜在的应用价值。