The decolorization of dye and textile effluent by Trametes hirsuta was studied in both induced and non-induced media. A removal of 70-100% of the color was achieved through adsorption and the action of laccases. Laccase activity was increased significantly with the addition of grapefruit peel (4000 U/mL) and effluent with grapefruit peel (16,000 U/mL) in comparison with the basal medium (50 U/mL). Analysis of the expression of laccase isoenzymes lac-B and lac-T revealed clear differences in the expression of these genes. The low levels of expression of lac-B in all media suggest a basal or constitutive gene expression, whereas lac-T was over-expressed in the media with effluent, and showed an up/down regulation depending on culture conditions and time. The results obtained suggest that the lac-T gene of T. hirsuta is involved in the decolorization of dyes.

中文翻译：

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毛栓菌Bm-2和lac-T作为可能的主要漆酶贡献基因对纺织废水的脱色

在诱导和非诱导介质中研究了毛栓菌对染料和纺织废水的脱色。通过吸附和漆酶的作用，去除了 70-100% 的颜色。与基础培养基 (50 U/mL) 相比，添加葡萄柚皮 (4000 U/mL) 和葡萄柚皮流出物 (16,000 U/mL) 后漆酶活性显着增加。漆酶同工酶 lac-B 和 lac-T 的表达分析揭示了这些基因表达的明显差异。所有培养基中 lac-B 的低表达水平表明是基础基因或组成型基因表达，而 lac-T 在有流出物的培养基中过度表达，并根据培养条件和时间显示出上调/下调。获得的结果表明 T.