Abstract

Crystals of mutant carboxypeptidase T from Thermoactinomyces vulgaris (CPT11QG) with amino-acid substitutions G215S, Q249G, A251G, T257A, D260G, T262D, and L254I and with the insertion ins253T were grown in microgravity by the capillary counter-diffusion method. The crystals belong to sp. gr. P31, which differs from the space group of the wild-type enzyme (P6322). The X-ray diffraction data set was collected from the crystals at the SPring-8 synchrotron facility (Japan) and is suitable for crystal structure determination at 2.45 Å resolution.

中文翻译：

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带有羧肽酶B主要特异性口袋和活性位点环的突变型羧肽酶T的制备，结晶和X射线衍射初步研究

摘要

通过毛细管反扩散法在微重力下生长具有氨基酸取代基G215S，Q249G，A251G，T257A，D260G，T262D和L254I的寻常型嗜热放线菌（CPT11QG）突变型羧肽酶T的晶体。晶体属于sp。gr。 P 31，不同于野生型酶的空间群（P 6322）。X射线衍射数据集是从日本SPring-8同步加速器设施的晶体中收集的，适合于以2.45Å分辨率进行晶体结构测定。