Identifying the extracellular metabolites of microorganisms in fresh vegetables is industrially useful for assessing the quality of processed foods. Pectobacterium carotovorum subsp. carotovorum (PCC) is a plant pathogenic bacterium that causes soft rot disease in cabbages. This microbial species in plant tissues can emit specific volatile molecules with odors that are characteristic of the host cell tissues and PCC species. In this study, we used headspace solid-phase microextraction followed by gas chromatography coupled with mass spectrometry (HS-SPME-GC-MS) to identify volatile compounds (VCs) in PCC-inoculated cabbage at different storage temperatures. HS-SPME-GC-MS allowed for recognition of extracellular metabolites in PCC-infected cabbages by identifying specific volatile metabolic markers. 4-Ethyl-5-methylthiazole and 3-butenyl isothiocyanate were identified as markers of fresh cabbages, whereas 2,3-butanediol and ethyl acetate were identified as markers of soft rot in PCC-infected cabbages. These analytical results demonstrate a suitable approach for establishing non-destructive plant pathogen-diagnosis techniques as alternatives to standard methods, within the framework of the development of rapid and efficient analytical techniques for monitoring plant-borne bacterial pathogens. These techniques also demonstrate promising applications for the freshness management and quality control of cabbages.

中文翻译：

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用于监测 Pectobacterium carotovorum 亚种的挥发性代谢标记物。carotovorum 使用顶空固相微萃取结合气相色谱-质谱法。

鉴定新鲜蔬菜中微生物的细胞外代谢物在工业上可用于评估加工食品的质量。Pectobacterium carotovorum亚种。胡萝卜素(PCC) 是一种植物病原菌，可引起卷心菜软腐病。植物组织中的这种微生物物种可以散发出具有宿主细胞组织和 PCC 物种特有气味的特定挥发性分子。在这项研究中，我们使用顶空固相微萃取，然后使用气相色谱联用质谱 (HS-SPME-GC-MS) 来鉴定不同储存温度下接种 PCC 的卷心菜中的挥发性化合物 (VC)。HS-SPME-GC-MS 允许通过识别特定的挥发性代谢标记物来识别 PCC 感染的卷心菜中的细胞外代谢物。4-乙基-5-甲基噻唑和 3-丁烯基异硫氰酸酯被确定为新鲜卷心菜的标志物，而 2,3-丁二醇和乙酸乙酯被确定为感染 PCC 的卷心菜中软腐病的标志物。这些分析结果表明，在开发用于监测植物传播的细菌性病原体的快速有效分析技术的框架内，建立非破坏性植物病原体诊断技术作为标准方法的替代方法是一种合适的方法。这些技术还展示了在卷心菜的新鲜度管理和质量控制方面很有前途的应用。