Most cervical cancers are associated with high-risk human papillomavirus (HPV) infection. Currently, cervical cancer treatment entails surgical removal of the lesion, but treatment of infection and preventing tissue damage are issues that still remain to be addressed. Herbal medicine and biological studies have focused on developing anti-viral drugs from natural sources. In this study, we analyzed the potential antiviral effects of Pinus densiflora Sieb. et Zucc. leaf extracts against HPV. The pine needle extracts from each organic solvent were analyzed for anti-viral activity. The methylene chloride fraction (PN-MC) showed the highest activity against HPV pseudovirus (PV). The PN-MC extract was more effective before rather than after treatment, and therefore represents a prophylactic intervention. Mice were pre-treated with PN-MC via genital application or oral administration, followed by a genital or subcutaneous challenge with HPV PV, respectively. The results of HPV challenge showed that mice treated via genital application showed complete protection against HPV. In conclusion, PN-MC represents a potential topical virucide for HPV infection.

中文翻译：

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松针提取物适用于预防人乳头瘤病毒感染的局部治疗。

大多数宫颈癌与高危人乳头瘤病毒 (HPV) 感染有关。目前，宫颈癌的治疗主要是手术切除病灶，但感染的治疗和组织损伤的预防仍是亟待解决的问题。草药和生物学研究的重点是从天然来源开发抗病毒药物。在这项研究中，我们分析了Pinus densiflora的潜在抗病毒作用锡布。等祖克。叶提取物抗 HPV。分析了每种有机溶剂的松针提取物的抗病毒活性。二氯甲烷部分 (PN-MC) 显示出对 HPV 假病毒 (PV) 的最高活性。PN-MC 提取物在治疗前比治疗后更有效，因此代表了预防性干预。通过生殖器应用或口服给药对小鼠进行 PN-MC 预处理，然后分别用 HPV PV 进行生殖器或皮下攻击。HPV 攻击的结果表明，通过生殖器应用治疗的小鼠显示出针对 HPV 的完全保护。总之，PN-MC 代表了一种潜在的 HPV 感染局部杀病毒剂。