Soil has been used for treatment of wound and skin diseases and for cosmetic purposes. Fusidium coccineum (FC) SA-1FC (Ascomycota) is a fungus found in nature, and its by-products are present in humid soils with plant humus. This study investigates the medium of fermented FC as a covering for all skin problems, including dryness, inflammation, and wounds. A preliminary study revealed that an alcohol extract of FC had a skin-enhancing effect, and thin-layer chromatography revealed a major component in a non-polar fraction. Here we identify a major compound isolated from a non-polar fraction as β-glucogallin. The mRNA levels of filaggrin and HAS3 are upregulated by FC and β-glucogallin treatment in keratinocytes and immortalized human keratinocytes cells. In addition, FC and β-glucogallin exert anti-inflammatory effects by suppressing expression of interleukin-4/poly(I:C)-induced chemokines and inflammatory cytokines. In fibroblasts, Hs68 cells, FC and β-glucogallin stimulate cell migration. These results suggest that FC and β-glucogallin can enhance skin barrier function.

中文翻译：

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球孢镰刀菌中的β-糖蛋白及其对皮肤屏障作用的增强

土壤已被用于治疗伤口和皮肤疾病以及用于美容目的。球孢镰刀菌（FC）SA-1FC（Ascomycota）是自然界中发现的一种真菌，其副产物存在于植物腐殖质的潮湿土壤中。这项研究调查了发酵的FC培养基是否可以覆盖所有皮肤问题，包括干燥，发炎和伤口。初步研究表明，FC的醇提取物具有增强皮肤的作用，而薄层色谱分析则表明非极性组分中的主要成分。在这里，我们确定了从非极性组分中分离出的主要化合物为β-葡萄糖醇。通过角质形成细胞和永生化的人角质形成细胞中的FC和β-葡萄糖gallin处理，丝蛋白和HAS3的mRNA水平被上调。此外，FC和β-葡萄糖gallin通过抑制白介素4 /聚（I：C）诱导的趋化因子和炎性细胞因子的表达发挥抗炎作用。在成纤维细胞中，Hs68细胞，FC和β-葡萄糖gallin刺激细胞迁移。这些结果表明，FC和β-葡萄糖gallin可以增强皮肤屏障功能。