Hollow colloidosomes with an enzyme confined in a porous shell as Pickering interfacial biocatalysts for efficient bioconversions,Green Chemistry

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Hollow colloidosomes with an enzyme confined in a porous shell as Pickering interfacial biocatalysts for efficient bioconversions
Green Chemistry ( IF 9.3 ) Pub Date : 2020-11-2 , DOI: 10.1039/d0gc02999h
Mengmeng Sun _{1,

2,

3,

4} , Wei Yin _{1,

2,

3,

4} , Jialin Chen _{1,

2,

3,

4} , Weihao Wang _{1,

2,

3,

4} , Ting Guo _{1,

2,

3,

4} , Tao Meng _{1,

2,

3,

4}

Affiliation

Lipase-loaded hollow colloidosomes are prepared for Pickering interfacial biocatalysis (PIB). Unlike in other systems, colloidosomes are self-assembled by lipases and silica nanoparticles, achieving a high enzyme-loading process without chemical crosslinking, and can stabilize Pickering emulsion due to the amphiphilic lipase confined in the porous shell. Remarkably, the enzyme encapsulation rate is 95.2%, which is better than other enzyme-loaded carriers in PIB reported so far. This system maintains 80.4% of the initial enzyme activity after 10 cycles, which provides a powerful and green platform for interfacial biocatalysis.

中文翻译：

具有限制在多孔壳中的酶的空心胶体，作为Pickering界面生物催化剂，可实现有效的生物转化

准备用于Pickering界面生物催化（PIB）的负载脂肪酶的空心胶体。与其他系统不同，胶体是通过脂肪酶和二氧化硅纳米粒子自组装的，无需化学交联即可实现高酶负载过程，并且由于封闭在多孔壳中的两亲脂肪酶可以稳定Pickering乳液。值得注意的是，该酶的包封率为95.2％，优于迄今为止报道的PIB中其他载有酶的载体。该系统在10个循环后仍可维持80.4％的初始酶活性，这为界面生物催化提供了强大而绿色的平台。

更新日期：2020-12-17

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