Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Serratia marcescens-derived fluorescent carbon dots as a platform toward multi-mode bioimaging and detection of p-nitrophenol
Analyst ( IF 3.6 ) Pub Date : 2020-10-30 , DOI: 10.1039/d0an01624a Yafang Ding 1, 2, 3, 4 , Wenzhang Tan 1, 2, 3, 4 , Xiaodan Zheng 1, 2, 3, 4 , Xiuling Ji 1, 2, 3, 4 , Pengfei Song 4, 5, 6, 7, 8 , Limei Bao 2, 3, 4, 9 , Chunting Zhang 1, 2, 3, 4 , Junjie Shang 1, 2, 3, 4 , Kunhao Qin 1, 2, 3, 4 , Yunlin Wei 1, 2, 3, 4
Analyst ( IF 3.6 ) Pub Date : 2020-10-30 , DOI: 10.1039/d0an01624a Yafang Ding 1, 2, 3, 4 , Wenzhang Tan 1, 2, 3, 4 , Xiaodan Zheng 1, 2, 3, 4 , Xiuling Ji 1, 2, 3, 4 , Pengfei Song 4, 5, 6, 7, 8 , Limei Bao 2, 3, 4, 9 , Chunting Zhang 1, 2, 3, 4 , Junjie Shang 1, 2, 3, 4 , Kunhao Qin 1, 2, 3, 4 , Yunlin Wei 1, 2, 3, 4
Affiliation
|
Carbon dots (CDs) have excellent application prospects in various fields such as fluorescent dyes, but expanding their application, especially in bioimaging and the detection of organic pollutants, is still a major research objective. In this study, fluorescent CDs were successfully synthesized via the hydrothermal method using Serratia marcescens KMR-3. The platform based on CDs-KMR3 exhibited excellent stability, good biocompatibility, and low biotoxicity, and can be effectively applied to the imaging of bacteria, fungi, plant cells, protozoa and mammalian cells, and can specifically stain the membranes of all tested cells. In this study, for the first time, bacteria-derived CDs were used to image the representative species of organisms ranging from lower-order to higher-order organisms, thereby proving the feasibility of the application of CDs in the fluorescence imaging of Paramecium caudatum. Additionally, CDs-KMR3 can rapidly diffuse into all the parts of the leaf through diffusion into the veins and intercellular interstitium in response to the induction of transpiration. Moreover, the data illustrate that CDs-KMR3 are likely to enter the digestive tracts of microworms by ingestion through the oral cavity and pharynx, and spread to the pseudocoelom and somatic cells, and finally to be excreted from microworms through the anus. Furthermore, this platform can be utilized as fluorescent probes for the rapid and highly selective detection of p-nitrophenol (p-NP). Moreover, this study contributed to the increased application of bacteria-derived CDs in bioimaging and detection of p-NP.
中文翻译：
粘质沙雷氏菌（Serratia marcescens）衍生的荧光碳点作为多模式生物成像和对硝基苯酚检测的平台
碳点（CD）在荧光染料等各个领域中都有极好的应用前景，但扩大其应用范围，尤其是在生物成像和有机污染物的检测中，仍然是主要的研究目标。在这项研究中，使用粘质沙雷氏菌通过水热方法成功合成了荧光CD。KMR-3。基于CDs-KMR3的平台表现出优异的稳定性，良好的生物相容性和低的生物毒性，可有效地应用于细菌，真菌，植物细胞，原生动物和哺乳动物细胞的成像，并可特异性染色所有测试细胞的膜。在这项研究中，首次使用细菌衍生的CD对从低阶到高阶生物的代表性生物成像，从而证明了CD在尾草履虫的荧光成像中应用的可行性。。另外，响应于蒸腾作用的诱导，CDs-KMR3可以通过扩散到静脉和细胞间质而迅速扩散到叶片的所有部分。此外，数据表明，CDs-KMR3可能通过食入通过口腔和咽部进入微蠕虫的消化道，并扩散到假体腔和体细胞，最后通过肛门从微蠕虫中排出。此外，该平台可用作荧光探针，用于快速，高度选择性地检测对硝基苯酚（p -NP）。此外，这项研究有助于增加细菌来源的CD在生物成像和p -NP检测中的应用。
更新日期：2020-12-09
中文翻译：
粘质沙雷氏菌（Serratia marcescens）衍生的荧光碳点作为多模式生物成像和对硝基苯酚检测的平台
碳点（CD）在荧光染料等各个领域中都有极好的应用前景，但扩大其应用范围，尤其是在生物成像和有机污染物的检测中，仍然是主要的研究目标。在这项研究中，使用粘质沙雷氏菌通过水热方法成功合成了荧光CD。KMR-3。基于CDs-KMR3的平台表现出优异的稳定性，良好的生物相容性和低的生物毒性，可有效地应用于细菌，真菌，植物细胞，原生动物和哺乳动物细胞的成像，并可特异性染色所有测试细胞的膜。在这项研究中，首次使用细菌衍生的CD对从低阶到高阶生物的代表性生物成像，从而证明了CD在尾草履虫的荧光成像中应用的可行性。。另外，响应于蒸腾作用的诱导，CDs-KMR3可以通过扩散到静脉和细胞间质而迅速扩散到叶片的所有部分。此外，数据表明，CDs-KMR3可能通过食入通过口腔和咽部进入微蠕虫的消化道，并扩散到假体腔和体细胞，最后通过肛门从微蠕虫中排出。此外，该平台可用作荧光探针，用于快速，高度选择性地检测对硝基苯酚（p -NP）。此外，这项研究有助于增加细菌来源的CD在生物成像和p -NP检测中的应用。
京公网安备 11010802027423号