Thyroid cancer is the most common endocrine cancer in the world. Noting that the NOS3 gene polymorphism interferes with nitric oxide production, this study aims to identify and analyze the NOS3 gene polymorphism in the intron 4 region in patients with papillary thyroid cancer. A case-control study was conducted with 31 papillary thyroid cancer patients of both genders who underwent thyroidectomy and treatment with sodium iodide radiopharmaceutical (131I) compared with 81 control patients. Through papillary thyroid cancer, the results were observed, compiled, and analyzed using SPSS version 25.0. The significance level of 5% was adopted. Genotypic frequencies of healthy subjects were in the Hardy-Weinberg equilibrium (P = 0.503). There was a significant genotypic difference between papillary thyroid cancer and healthy individuals (P <0.001). The BB genotype conferred a protective factor for papillary thyroid cancer (P <0.001, odds ratio (OR) 0.16; 95% confidence interval (CI) 0.06, 0.42), while the presence of the A allele appears to be a risk factor for papillary thyroid cancer (P <0.001, OR 3.54; 95% CI 1.86, 6.73). The intron 4 polymorphism of the NOS3 gene was associated with susceptibility to papillary thyroid cancer. Thus, future research into the effects of this polymorphism is essential.

甲状腺癌是世界上最常见的内分泌癌。注意到NOS3基因多态性干扰一氧化氮的产生，本研究旨在鉴定和分析甲状腺乳头状癌患者内含子4区的NOS3基因多态性。对 31 名接受甲状腺切除术和碘化钠放射性药物 (131I) 治疗的两性甲状腺乳头状癌患者与 81 名对照患者进行了病例对照研究。通过乳头状甲状腺癌，使用SPSS 25.0版本对结果进行观察、整理和分析。采用 5% 的显着性水平。健康受试者的基因型频率处于 Hardy-Weinberg 平衡（P= 0.503）。甲状腺乳头状癌与健康个体之间存在显着的基因型差异（P <0.001）。BB 基因型赋予乳头状甲状腺癌的保护因素（P <0.001，优势比（OR）0.16；95% 置信区间（CI）0.06，0.42），而 A 等位基因的存在似乎是乳头状甲状腺癌的危险因素甲状腺癌（P <0.001，OR 3.54；95% CI 1.86，6.73）。NOS3基因的内含子 4 多态性与甲状腺乳头状癌的易感性有关。因此，未来研究这种多态性的影响是必不可少的。