ABSTRACT Nicotinamide adenine dinucleotide (NAD)/NAD phosphate (NADPH) is essential for numerous redox reactions and serve as co-factors in multiple metabolic processes in all organisms. NAD kinase (NADK) is an enzyme involved in the synthesis of NADP+ from NAD+ and ATP. Arabidopsis NADK2 (AtNADK2) is a chloroplast-localizing enzyme that provides recipients of reducing power in photosynthetic electron transfer. When Arabidopsis plants were grown on MS medium supplemented with 5 mM MgSO4, an AtNADK2-overexpressing line exhibited higher glutathione and total sulfur accumulation than control plants. Metabolomic analysis of major amino acids and organic acids using capillary electrophoresis–mass spectrometry demonstrated that overexpression of AtNADK2 affected a range of metabolic processes in response to MgSO4 supplementation.

中文翻译：

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补充硫酸镁改变叶绿体 NAD 激酶过表达拟南芥的代谢

摘要 烟酰胺腺嘌呤二核苷酸 (NAD)/NAD 磷酸盐 (NADPH) 是众多氧化还原反应必不可少的，并且在所有生物体的多种代谢过程中充当辅助因子。NAD 激酶 (NADK) 是一种参与从 NAD+ 和 ATP 合成 NADP+ 的酶。拟南芥 NADK2 (AtNADK2) 是一种叶绿体定位酶，可在光合电子转移中为受体提供还原能力。当拟南芥植物在补充有 5 mM MgSO4 的 MS 培养基上生长时，AtNADK2 过表达株系表现出比对照植物更高的谷胱甘肽和总硫积累。使用毛细管电泳-质谱法对主要氨基酸和有机酸的代谢组学分析表明，AtNADK2 的过表达影响了一系列代谢过程，以响应 MgSO4 补充。