Abstract

Given that some Verbascum species are effective against influenza viruses, an endemic species, Verbascum pseudoholotrichum is thought to have the potential to be used in the treatment of virucidal diseases. It was extensively investigated for its antioxidant and antimicrobial activities, chemical and trace element composition. The soxhlet extraction method was applied in the extraction process of the above-ground parts of the plant using three solvents (methanol, ethanol and water). The total phenolic content of extracts was between 175.7 and 186.1 mg GAE/100 g on the dried weight basis. DPPH radical scavenging activity analysis was performed and IC₅₀ values were 0.539–1.254 mg mL⁻¹. In total, 38 compounds were detected by GC–MS analysis. ICP-MS analysis was applied to identify the total contents of 24 elements in dried samples. The antimicrobial activity of the extracts was evaluated on six different pathogens using the spectrophotometric microdilution technique. The obtained minimum inhibitory concentration values were 17.37 µ–113.9 µg mL⁻¹ at the end of the 24-h incubation. The highest and lowest inhibition zones were 5.0 and 0.3 mm for ethanol extract against Bacillus subtilis and water extract against Enterococcus fecalis, respectively, and no resistance was seen against Escherichia coli using disc diffusion method.

摘要

鉴于某些毛蕊花对流感病毒（一种地方性物种）有效，因此认为毛蕊花具有用于治疗病毒性疾病的潜力。它因其抗氧化和抗菌活性、化学和微量元素组成而被广泛研究。索氏提取法应用于植物地上部分的提取过程，使用三种溶剂（甲醇、乙醇和水）。提取物的总酚含量在 175.7 和 186.1 毫克 GAE/100 克干重之间。进行了DPPH自由基清除活性分析，IC ₅₀值为0.539-1.254 mg mL ^-1. 通过 GC-MS 分析总共检测到 38 种化合物。ICP-MS 分析用于鉴定干燥样品中 24 种元素的总含量。使用分光光度微量稀释技术评估了提取物对六种不同病原体的抗菌活性。在 24 小时孵育结束时，获得的最小抑制浓度值为 17.37 µ–113.9 µg mL ^{-1 。}乙醇提取物对枯草芽孢杆菌和水提取物对粪肠球菌的最高和最低抑菌圈分别为5.0和0.3 mm，盘扩散法对大肠杆菌没有耐药性。