ABSTRACT

There is an increased public concern about potential health hazards of exposure to electromagnetic radiation (EMR). To declare the carcinogenic effects of 1800 MHz EMR. In this study, Balb/c-3T3 cells were exposed to 1800 MHz EMR for 80 days. The cells were harvested for cell proliferation detection, cell cycle assay, plate clone, and soft agar formation assay, transwell assay, and mRNA microarray detection. 1800 MHz EMR promoted Balb/c-3T3 proliferation. No clones were observed in both plate clone and soft agar clone formation assay. The percentage of cells in S phase in Balb/c-3T3 cells of 80d Expo was obviously higher than the percetage in 80d Sham cells. 80d Expo Balb/c-3T3 cells had stronger migration ability than Sham cells. The mRNA microarray results indicated that cell cycle, cell division, and DNA replication were the main biological processes the significant genes enriched, with higher expression of RPs and Mcms. 1800 MHz EMR promoted Balb/c-3T3 cells proliferation and migration. The mRNA microarray results indicated that cell cycle, cell division, and DNA replication were the main biological processes the significant genes enriched.

摘要

公众越来越担心暴露于电磁辐射（EMR）对健康的危害。宣布1800 MHz EMR的致癌作用。在这项研究中，Balb / c-3T3细胞暴露于1800 MHz EMR 80天。收获细胞用于细胞增殖检测，细胞周期测定，平板克隆和软琼脂形成测定，transwell测定和mRNA微阵列检测。1800 MHz EMR促进了Balb / c-3T3增殖。在平板克隆和软琼脂克隆形成测定中均未观察到克隆。80d Expo Balb / c-3T3细胞S期细胞百分比明显高于80d Sham细胞百分率。80d Expo Balb / c-3T3细胞比Sham细胞具有更强的迁移能力。mRNA微阵列结果表明，细胞周期，细胞分裂，DNA复制和DNA复制是重要基因富集的主要生物学过程，其中RPs和Mcms的表达较高。1800 MHz EMR促进Balb / c-3T3细胞增殖和迁移。mRNA微阵列结果表明，细胞周期，细胞分裂和DNA复制是重要基因富集的主要生物学过程。