Abstract

The sorting nexin 29 gene (SNX29) is a well-known regulator of myocyte differentiation and proliferation. In this work, two indels (17-bp and 21-bp) were identified in the goat SNX29 gene, and their effects on the growth traits of 1,759 Shaanbei white cashmere (SBWC) goats were analyzed. Both indels had three genotypes [homozygote wild type (II), heterozygote (ID), and homozygote mutation (DD)] and displayed medium genetic diversity (0.25 < polymorphism information content (PIC) < 0.50) in the population. The 17-bp indel was significantly associated with chest width (p = 0.009), body weight (p = 0.021), and chest depth (p = 0.032), with the II genotype dominant. The 21-bp indel was significantly associated with chest width (p = 0.001), chest depth (p = 4.8E-5), heart girth (p = 0.007), and hip width (p = 0.002). Because the two indels were in the upstream (17-bp) and intron (21-bp) regions of the SNX29 gene, transcription factor binding sites were predicted. The IRF5 and MYC could bind with the 17-bp indel and 21-bp indel sequences, respectively. This study indicates that SNX29 is a promising candidate gene that can be used to improve meat production in goat breeding.

摘要

分选 nexin 29 基因 ( SNX29 ) 是众所周知的肌细胞分化和增殖调节因子。在这项工作中，在山羊SNX29基因中鉴定了两个插入缺失（17-bp 和 21-bp），并分析了它们对 1,759 只陕北白绒 (SBWC) 山羊生长性状的影响。两个插入缺失都具有三种基因型[纯合子野生型 (II)、杂合子 (ID) 和纯合子突变 (DD)]，并在人群中显示出中等遗传多样性 (0.25 < 多态性信息含量 (PIC) < 0.50)。17 bp 插入缺失与胸宽 ( p  = 0.009)、体重 ( p  = 0.021) 和胸深 ( p = 0.032)，II 基因型占主导地位。21-bp 插入缺失与胸宽 ( p  = 0.001)、胸深 ( p  = 4.8E-5)、心围 ( p  = 0.007) 和臀宽 ( p  = 0.002) 显着相关。由于两个插入缺失位于SNX29基因的上游 (17-bp) 和内含子 (21-bp) 区域，因此预测了转录因子结合位点。IRF5 和 MYC 可以分别与 17-bp 插入缺失和 21-bp 插入缺失序列结合。该研究表明，SNX29是一个很有前途的候选基因，可用于提高山羊育种的肉类产量。