Abstract

Understanding the roles of the properties of nanomaterials in biological interactions is a key issue in their safe applications, but the surface atomic arrangement, as an important property of engineered nanomaterials (ENMs), remains largely unknown. Herein, the interfacial interactions (affinity sites and intensity) between monolayer MoS₂ and zebrafish embryos mediated by 1 T phase surface atomic arrangement (octahedral coordination) and the 2H phase surface atomic arrangement (triangular prism coordination) MoS₂ nanosheets were studied. 1 T-MoS₂ first bound to phosphate and then proteins on the chorion, while the adhesion of 2H-MoS₂ occurred in the opposite order. The binding affinity of 2H-MoS₂ with embryos was higher than that of 1 T-MoS₂, and the former material changed the protein structure from β-sheets to turns and bends and random coils. Compared to 1 T-MoS₂, 2H-MoS₂ more readily entered embryos, which was facilitated by caveolae-mediated endocytosis, and caused higher developmental toxicity. Furthermore, metabolic pathways related to amino acid and protein biosynthesis and energy metabolism were affected by the nanomaterial surface atomic arrangements. The above results provide insights into the designs, applications and risk assessments of nanomaterials by the surface atomic arrangement regulation.

抽象的

理解纳米材料的特性在生物相互作用中的作用是其安全应用中的关键问题，但是作为工程纳米材料（ENM）的重要特性，表面原子排列仍是未知之数。在本文中，研究了由1 T相表面原子排列（八面体配位）和2H相表面原子排列（三角形棱柱配位）MoS ₂纳米片介导的单层MoS ₂和斑马鱼胚胎之间的界面相互作用（亲和力和强度）。1 T-MoS ₂首先与磷酸盐结合，然后与绒毛膜上的蛋白质结合，而2H-MoS ₂的粘附则以相反的顺序发生。2H-MoS ₂的结合亲和力带有胚胎的蛋白质比1 T-MoS ₂的蛋白质要高，并且前一种材料将蛋白质结构从β-折叠层变成了转弯，弯曲和无规卷曲。与1 T-MoS ₂相比，2H-MoS ₂更容易进入胚胎，这是由小窝介导的内吞作用促进的，并引起更高的发育毒性。此外，与氨基酸和蛋白质生物合成以及能量代谢有关的代谢途径受到纳米材料表面原子排列的影响。以上结果通过表面原子排列规则为纳米材料的设计，应用和风险评估提供了见识。