ABSTRACT

Background: LOY is associated with ageing and increase the incidence of cancers.

Aims: To elucidate the role of LOY in various cancer types, namely, prostate (PRT), pancreatic (PC), and colorectal (CRC) cancers in males.

Material and Methods: Fifty CRC patients [mean age = 44.58±11.2 years], fifty PRT [mean age= 60.48± 17.07 years] and fifty PC [mean age = 48.74 ±16.45 years] along with 100 healthy controls [mean age= 54.06 ±15.04 years] were recruited. DNA was isolated from peripheral blood and was subjected to multiplex QF-PCR. The Y/X ratio was calculated from the peak height.

Results: The mean Y/X ratio was lower in all patients with cancers (0.875333± 0.086; p value˂ 0.0001) than in controls (1.11 ± 0.071), as well as, in CRC (0.926±0.192; p value˂0.0001), PC (0.85 ± 0.0311; p value˂0.0001) and PRT (0.85±0.122; p value˂0.0001) when calculated separately. Multivariate logistic regression analysis was used to analyze the strength of the presence of cancer prediction using the percentage of LOY and age showed that LOY (p= 0.001) is a better predictor of cancer presence than age (p= 0.359).

Conclusion: LOY in blood could be a predictive biomarker in the carcinogenesis of males.

摘要

背景： LOY 与衰老有关并增加癌症的发病率。

目的：阐明 LOY 在各种癌症类型中的作用，即男性的前列腺癌 (PRT)、胰腺癌 (PC) 和结直肠癌 (CRC)。

材料和方法：50 名 CRC 患者 [平均年龄 = 44.58±11.2 岁]、50 名 PRT [平均年龄 = 60.48±17.07 岁]和 50 名 PC [平均年龄 = 48.74 ±16.45 岁]以及 100 名健康对照 [平均年龄 = 54.06] ±15.04岁]被招募。从外周血中分离 DNA 并进行多重 QF-PCR。Y/X 比值由峰高计算。

结果：所有癌症患者的平均 Y/X 比值 (0.875333±0.086；p 值˂ 0.0001) 低于对照组 (1.11±0.071)，以及 CRC (0.926±0.192；p 值˂0.0001) , PC (0.85 ± 0.0311; p value˂0.0001) 和 PRT (0.85±0.122; p value˂0.0001) 分别计算。多变量逻辑回归分析用于使用 LOY 的百分比和年龄来分析癌症预测的强度，表明 LOY (p = 0.001) 是比年龄 (p = 0.359) 更好的癌症存在预测因子。

结论：血液中的 LOY 可能是男性癌症发生的预测生物标志物。