ABSTRACT: Phytoene and astaxanthin are 2 important carotenoids in the green alga Haematococcus pluvialis. Under environmental stress, the synthesis of phytoene in H. pluvialis increases significantly, and phytoene is converted to astaxanthin through enzymatic catalysis. This paper analyzes the relationship between astaxanthin and phytoene accumulation in carotenoid synthesis pathways under different concentrations of sodium acetate (NaAc) by high-performance liquid chromatography. The highest concentrations of phytoene and astaxanthin were observed at the NaAc concentration of 6 g l^-1 on the 12^th day of induction. The highest astaxanthin concentration achieved was 2.26 ± 0.28%. Therefore, we concluded that 6 g l^-1 NaAc and induction for 12 d provided the optimal inducing conditions for astaxanthin accumulation in H. pluvialis. psy, pds, lcyB, β-carotene ketolase crtw, and crtz, which are genes related to phytoene and astaxanthin synthesis, were cloned and studied at the transcriptional level. crtw and crtz were continuously up-regulated since the first day of induction, while psy, pds, and lcyB were continuously up-regulated starting on the 3^rd day of induction. These findings are important for enhancing our understanding of the mechanism of accumulation of phytoene and astaxanthin in H. pluvialis and provide a foundation for identifying the induction conditions necessary for optimizing astaxanthin production and increasing astaxanthin yields.

中文翻译：

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乙酸钠胁迫下雨生红球菌中植酸和虾青素的积累及相关基因的表达

摘要：番茄红素和虾青素是绿藻血球菌中的两种重要的类胡萝卜素。下的环境压力，在八氢番茄红素合成H.生红球藻的增加显著，八氢番茄红素和通过酶催化转化为虾青素。本文通过高效液相色谱法分析了不同浓度的乙酸钠（NaAc）下虾青素与类胡萝卜素合成途径中的八氢番茄红素积累之间的关系。在诱导的^第12天，在NaAc浓度为6 gl ^-1时观察到了最高的植物六氢番茄红素和虾青素浓度。达到的最高虾青素浓度为2.26±0.28％。因此，我们得出结论6 gl ^-1NaAc和诱导12 d为雨生红杆菌积累虾青素提供了最佳诱导条件。克隆和研究了与植物生长素和虾青素合成相关的基因 psy， pds，lcyB，β-胡萝卜素酮醇酶crtw和crtz，并在转录水平进行了研究。crtw和的crtZ连续自感应的第一天上调，而 PSY， PDS和lcyB连续上调开始在3^次上岗日。这些发现对增进我们对红藻中植酸和虾青素积累机制的理解具有重要意义，并为确定优化虾青素产量和增加虾青素产量所必需的诱导条件提供了基础。