Identification of Candida auris is challenging and requires molecular or protein profiling-based approaches, availability of which is limited in many routine diagnostic laboratories, necessitating the development of a cost-effective, rapid, and reliable method of identification. The objective of this study was to develop a selective medium for C. auris identification. Eighteen C. auris and 30 non-C. auris yeasts were used for the standardization of the selective medium. Sodium chloride (10% to 13% concentration) and ferrous sulfate (8 mM to 15 mM) were added to yeast extract-peptone-dextrose (YPD) agar in various combinations followed by incubation at 37°C, 40°C, or 42°C for 2 to 3 days. For validation, 579 yeast isolates and 40 signal-positive Bactec blood culture (BC) broths were used. YPD agar comprising 12.5% NaCl and 9 mM ferrous sulfate incubated at 42°C for 48 h, named Selective Auris Medium (SAM), allowed selective growth of C. auris. A total of 95% (127/133) of C. auris isolates tested grew on the standardized media within 48 h, and the remaining 6 isolates grew after 72 h, whereas the growth of 446 non-C. auris yeast isolates was completely inhibited. The specificity and sensitivity of the test medium were both 100% after 72 h of incubation. The positive and negative predictive values were also noted to be 100% after 72 h of incubation. The formulated selective medium can be used for the detection and identification of C. auris. The SAM is inexpensive, can easily be prepared, and can be used as an alternative to molecular diagnostic tools in the clinical microbiology laboratory.

中文翻译：

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一种用于分离和检测病原菌的念珠菌的选择性培养基

鉴定假丝酵母具有挑战性，并且需要基于分子或蛋白质谱的方法，在许多常规诊断实验室中其可用性受到限制，因此必须开发一种经济高效，快速且可靠的鉴定方法。这项研究的目的是开发一种用于鉴定金黄色葡萄球菌的选择性培养基。18个金龟和30个非金龟酵母用于选择性培养基的标准化。将氯化钠（浓度为10％至13％）和硫酸亚铁（浓度为8 mM至15 mM）以各种组合添加到酵母提取物-蛋白糊-葡萄糖（YPD）琼脂中，然后在37°C，40°C或42°C下孵育°C 2至3天。为了进行验证，使用了579个酵母菌分离物和40个信号阳性的Bactec血液培养（BC）肉汤。包含12.5％NaCl和9 mM硫酸亚铁的YPD琼脂在42°C下孵育48小时，命名为Selective Auris Medium（SAM），可选择性培养金黄色梭菌。共有的95％（133分之127）C.耳分离测试的生长在标准介质48小时内，并且剩余的6株72小时后的增长，而446个非生长C.耳酵母分离株被完全抑制。温育72小时后，测试培养基的特异性和敏感性均为100％。孵育72小时后，阳性和阴性预测值也被认为是100％。配制的选择性培养基可用于检测和鉴定金龟子。SAM价格便宜，易于制备，并可在临床微生物学实验室中用作分子诊断工具的替代品。