Aedes japonicus japonicus (Theobald) is a relatively recent immigrant to the Pacific Northwest, having been collected in Washington State in 2001 and in British Columbia (BC) since 2014. We applied a molecular barcoding approach to determine the phylogenetic relationship of Ae. j. japonicus populations in BC with those from around the world. We sequenced a 617 base-pair segment of the cytochrome c oxidase 1 gene and a 330 base-pair region of the NADH dehydrogenase 4 gene to find genetic variation and characterize phylogenetic and haplotypic relationships based on nucleotide divergences. Our results revealed low genetic diversity in the BC samples, suggesting that these populations arose from the same introduction event. However, our approach lacked the granularity to identify the exact country of origin of the Ae. j. japonicus collected in BC. Future efforts should focus on detecting and preventing new Ae. j. japonicus introductions, recognizing that current molecular techniques are unable to pin-point the precise source of an introduction.

中文翻译：

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使用线粒体 DNA 研究加拿大不列颠哥伦比亚省引进的日本伊蚊（双翅目：Culicidae）种群的分子关系

Aedes japonicus japonicus (Theobald) 是太平洋西北部相对较新的移民，于 2001 年在华盛顿州和自 2014 年以来在不列颠哥伦比亚省 (BC) 收集。我们应用分子条形码方法来确定Ae 的系统发育关系。j. 刺参卑诗省的人口与来自世界各地的人口。我们对细胞色素 c 氧化酶 1 基因的 617 个碱基对片段和 NADH 脱氢酶 4 基因的 330 个碱基对区域进行测序，以发现遗传变异并基于核苷酸差异表征系统发育和单倍型关系。我们的结果显示 BC 样本中的遗传多样性较低，这表明这些种群来自同一引入事件。然而，我们的方法缺乏确定Ae确切原产国的粒度。j. BC 采集的日本粳稻。未来的努力应该集中在检测和预防新的Ae 上。j. japonicus介绍，认识到当前的分子技术无法确定介绍的精确来源。