Induced genetic ablation of Rest leads to the alteration of stimulus‐induced response of the vagal nerve,Genes to Cells

当前位置： X-MOL 学术 › Genes Cells › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Induced genetic ablation of Rest leads to the alteration of stimulus‐induced response of the vagal nerve
Genes to Cells ( IF 1.3 ) Pub Date : 2020-11-19 , DOI: 10.1111/gtc.12819
Hitomi Aoki ₁ , Chikara Abe ₂ , Akira Hara ₃ , Tatsuhiko Miyazaki ₄ , Hironobu Morita ₂ , Takahiro Kunisada ₁

Affiliation

Rest (RE1‐silencing transcription factor, also called Nrsf) is involved in the maintenance of the undifferentiated state of neuronal stem/progenitor cells by preventing precocious expression of neuronal genes. In order to further investigate the function of Rest in neurons, we generated and examined mice evoking genetic ablation of Rest specifically in neural tissues by generating Rest conditional knockout mice. As the Rest knockout mice are embryonically lethal, we used a Sox1‐Cre allele to excise the floxed Rest gene from the early stage of nerve cell differentiation including neural crest‐derived nerve cells. Using this conditional Rest knockout Sox1‐Cre; Rest^flox/flox mice, we have revealed the role of Rest in the parasympathetic nervous system in the stomach and heart

中文翻译：

诱导的休息基因消融导致迷走神经刺激诱导反应的改变

Rest（RE1沉默转录因子，也称为Nrsf）通过防止神经元基因的早熟表达来维持神经元干/祖细胞的未分化状态。为了进一步研究Rest在神经元中的功能，我们通过生成Rest条件敲除小鼠，生成并检查了在神经组织中引起Rest基因消融的小鼠。由于Rest基因敲除小鼠具有胚胎致死性，因此我们使用Sox1-Cre等位基因从神经细胞分化的早期阶段（包括神经嵴衍生神经细胞）中切除 floxed Rest基因。使用这种条件休息敲除Sox1-Cre； Rest ^flox/flox小鼠，我们揭示了Rest在胃和心脏副交感神经系统中的作用

更新日期：2020-11-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11