White tail disease (WTD) is a disease of Macrobrachium rosenbergii caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) with the potential to devastate the aquaculture industry. The present study aimed to explore the possible protection of M. rosenbergii against the disease by oral administration of bacterially expressed recombinant capsid proteins of MrNV and XSV. Juvenile M. rosenbergii were fed with the feed coated with inactivated bacteria encapsulated expressed recombinant viral proteins either individually or in combination for 7 days. Challenge studies using WTD causing agents were carried out after 3 (group I), 10 (group II) and 20 (group III) days post‐feeding of viral proteins. Recombinant capsid protein of MrNV showed better protection when compared to other treatments with relative per cent survival of 62.5% (group I), 57.9% (group II) and 39.5% (group III). Treatment controls of groups I, II and III showed 100%, 95% and 95% mortality, respectively. The study demonstrates that oral administration of recombinant capsid proteins of MrNV and XSV provides effective protection against WTD in freshwater prawn.

中文翻译：

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通过灭活细菌细胞口服递送的重组病毒蛋白可在罗氏沼虾（德文氏）中预防白尾病

白尾病（WTD）是一种疾病罗氏沼虾所致罗氏沼虾诺达（先生NV）和额外的小病毒（XSV）与蹂躏着养殖业的潜力。旨在探索可能的保护本研究罗氏沼虾由细菌表达的重组衣壳蛋白的口服抗疾病先生NV和XSV。罗氏沼虾少年分别用包被表达的灭活细菌包被的饲料喂食7天，所述灭活细菌包被表达的重组病毒蛋白。喂食病毒蛋白后第3天（第I组），第10天（第II组）和第20天（第III组）进行了使用WTD引起剂的攻击研究。与其他治疗方法相比，NV Mr的重组衣壳蛋白显示出更好的保护作用，相对存活率分别为62.5％（I组），57.9％（II组）和39.5％（III组）。I，II和III组的治疗对照组分别显示出100％，95％和95％的死亡率。该研究表明，口服施用NV和XSV先生的重组衣壳蛋白可有效抵抗淡水虾的WTD。