Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy,ChemBioChem

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Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy
ChemBioChem ( IF 2.6 ) Pub Date : 2020-11-18 , DOI: 10.1002/cbic.202000727
Jonathan Schwach ₁ , Ksenia Kolobynina ₂ , Katharina Brandstetter ₁ , Marcus Gerlach ₃ , Philipp Ochtrop ₄ , Jonas Helma ₃ , Christian P R Hackenberger _{4,

5} , Hartmann Harz ₁ , M Cristina Cardoso ₂ , Heinrich Leonhardt ₁ , Andreas Stengl ₁

Affiliation

Protein‐oligonucleotide conjugates. We present a novel strategy for the efficient and site‐specific generation of nanobody‐oligonucleotide conjugates in a 1 : 1 stoichiometry by Tub‐tag‐mediated conjugation. We show a proof‐of‐concept that these conjugates can readily be used for reversible staining in confocal fluorescence microscopy.