Dysregulation of pseudogenes is involved in the progression of various types of cancer, including glioblastoma (GBM). Proliferation associated-2G4 (PA2G4) pseudogene 4 (PA2G4P4) has been shown to play an oncogenic role in bladder cancer development. Our study aimed to explore the role and mechanism of PA2G4P4 in GBM progression. PA2G4P4 and PA2G4 expression in GBM tissues was analyzed using the GEPIA database. Cell viability, apoptosis, and activities of caspase-3 and caspase-9 in GBM cells were explored by CCK-8, flow cytometry analysis, and colorimetric activity assay kits, respectively. GEPIA database showed that PA2G4P4 and PA2G4 were both upregulated in GBM tissues. PA2G4P4 expression was also boosted in GBM cells. Knockdown of PA2G4P4 or PA2G4 inhibited cell viability, induced apoptosis, and increased caspase-3 and caspase-9 activities in GBM cells. Data from UALCAN database showed that among top 15 genes correlated with PA2G4P4, PA2G4 had the highest correlation coefficient. Additionally, knockdown of PA2G4P4 inhibited PA2G4 expression and nuclear translocation in GBM cells. Overexpression of PA2G4 abolished the functions of PA2G4P4 knockdown on viability and apoptosis in GBM cells. Summarily, pseudogene PA2G4P4 promotes oncogene PA2G4 expression and nuclear translocation to affect cell viability and apoptosis in GBM cells.

假基因的失调与包括胶质母细胞瘤（GBM）在内的各种类型癌症的发展有关。增殖相关的2G4（PA2G4）假基因4（PA2G4P4）已显示在膀胱癌的发展中具有致癌作用。我们的研究旨在探讨PA2G4P4在GBM进展中的作用和机制。使用GEPIA数据库分析GBM组织中的PA2G4P4和PA2G4表达。通过CCK-8，流式细胞术分析和比色活性检测试剂盒分别研究了GBM细胞中的细胞活力，凋亡以及caspase-3和caspase-9的活性。GEPIA数据库显示，GBM组织中PA2G4P4和PA2G4均上调。在GBM细胞中，PA2G4P4表达也得到了增强。敲低PA2G4P4或PA2G4抑制细胞活力，诱导细胞凋亡，并增加GBM细胞中的caspase-3和caspase-9活性。UALCAN数据库的数据显示，在与PA2G4P4相关的前15个基因中，PA2G4具有最高的相关系数。另外，敲低PA2G4P4抑制了GBM细胞中PA2G4的表达和核易位。PA2G4的过表达消除了PA2G4P4敲低对GBM细胞活力和凋亡的功能。综上所述，假基因PA2G4P4促进癌基因PA2G4的表达和核易位，从而影响GBM细胞的细胞活力和细胞凋亡。PA2G4的过表达消除了PA2G4P4敲低对GBM细胞活力和凋亡的功能。综上所述，假基因PA2G4P4促进癌基因PA2G4的表达和核易位，从而影响GBM细胞的细胞活力和细胞凋亡。PA2G4的过表达消除了PA2G4P4敲低对GBM细胞活力和凋亡的功能。综上所述，假基因PA2G4P4促进癌基因PA2G4的表达和核易位，从而影响GBM细胞的细胞活力和细胞凋亡。