Modification of exoelectrogens with conjugated polymers is an efficient strategy to improve extracellular electron transfer (EET) from individual cells to electrode. However, due to the hydrophobic nature of conductive polymers, improving cell-electrode adhesion needs to be addressed. In this research, we report the modification of Shewanella oneidensis MR-1 by successively in situ coating individual cells with polypyrrole (PPy) and adhesive polydopamine (PDA). The PPy-PDA modified cells display improved conductivity and adhesion. We employ PPy-PDA modified cells as anode in microbial fuel cells (MFCs) and find that electro-activity of anode is greatly improved as direct EET is improved, and riboflavin secretion which is conducive to indirect EET is enhanced. The maximum power density of MFCs employing PPy-PDA modified cells is 11.8 and 4.8 higher than that of PPy modified and unmodified cells, respectively. Our results indicate this in situ modification of exoelectrogens with PPY-PDA offers a facile and promising strategy for performance improvement of MFCs.

用共轭聚合物修饰外生电子是改善从单个细胞到电极的细胞外电子转移（EET）的有效策略。然而，由于导电聚合物的疏水性，需要解决改善电池-电极粘附的问题。在这项研究中，我们报道了Shewanella oneidensis的修饰MR-1通过依次用聚吡咯（PPy）和粘合性聚多巴胺（PDA）原位涂覆单个细胞来实现。PPy-PDA修饰的电池显示出改善的导电性和粘附性。我们在微生物燃料电池（MFCs）中采用PPy-PDA修饰的电池作为阳极，发现随着直接EET的提高，阳极的电活性大大提高，并且有助于间接EET的核黄素分泌得到增强。使用PPy-PDA修饰的单元的MFC的最大功率密度分别比PPy修饰的和未修饰的单元的最大功率密度高11.8和4.8。我们的结果表明，用PPY-PDA原位修饰外生电子为MFC的性能改进提供了一种简便而有希望的策略。