Considering pH-dependent fluorescence of curcuminoids, a microemulsion electrokinetic chromatographic (MEEKC) method was developed under acidic conditions for their separation and detection using laser-induced native fluorescence (LINF), so as to solve the analysis of urine metabolism for curcuminoids. The microemulsion composition was optimized by response surface methodology (RSM), and the effects of buffer pH and organic modifiers were systematically investigated. The optimal buffer for the separation of curcuminoids was chosen as follows: 2.8% (v/v) ethyl acetate, 80 mM SDS and 2.8% (v/v) n-butanol to form microemulsion, 28% (v/v) ethanol as organic modifier, and 20 mM phosphoric acid as electrolyte at pH 3.0. Under these conditions, four curcuminoids including curcumin, demethoxy curcumin (DMC), bisdemethoxy curcumin (BDMC) and demethyl curcumin (DEC) could be well separated within 18 min, and the detection limits (LOD, based on S/N=3) were calculated to be 71, 60, 22, and 147 pg mL⁻¹, respectively. Combined with solid-phase extraction (SPE), the developed MEEKC-LINF method has been successfully applied to continuously monitor the curcuminoids and related metabolites in human urine collected from a healthy volunteer after oral administration of curry, testifying that this method has potential for evaluating the pharmacological activity of curcuminoids.

考虑到姜黄素的pH依赖性荧光，开发了一种酸性条件下的微乳电动色谱法（MEEKC），用于分离和检测激光诱导的天然荧光（LINF），从而解决了尿中对姜黄素代谢的分析问题。通过响应面法（RSM）优化了微乳液的组成，并系统研究了缓冲液pH和有机改性剂的影响。选择用于分离姜黄素的最佳缓冲液如下：2.8％（v / v）乙酸乙酯，80 mM SDS和2.8％（v / v）正丁醇以形成微乳液，28％（v / v）乙醇有机改性剂和20 mM磷酸作为电解质，pH为3.0。在这些条件下，四种姜黄素包括姜黄素，脱甲氧基姜黄素（DMC），^-1。结合固相萃取（SPE），已开发的MEEKC-LINF方法已成功应用于连续监测从健康志愿者口服咖喱后收集的人尿中的姜黄素和相关代谢产物，证明该方法具有评估的潜力。姜黄素的药理活性。