In this study, surface-enhanced Raman scattering (SERS) based field-deployable platform has been explored for early detection and distinction of the human immunodeficiency virus (HIV-1). A highly optimized silver nanorods array, fabricated using glancing angle deposition technique was used as SERS substrate. Distinct signature peaks for varying concentrations (10² to 10⁶ copies/ml) were identified in five different HIV-1 subtypes (A, B, C, D, and CRF02_AG). Binding of viruses directly with Ag nanorods without using antibodies or intermediate reagents is shown. The purified viruses were spiked in water and healthy plasma to capture pure HIV-1 peaks. Distinct peaks were also captured for the X4 and R5 tropic strains suggesting tropism based detection. The above data was further confirmed and analyzed statistically using a multivariate tool. Thus, the present study indicates the ability of the SERS platform to detect and differentiate the HIV-1 virus implying its further validation using clinical specimens and isolates.

在这项研究中，已经探索了基于表面增强拉曼散射（SERS）的现场可部署平台，用于早期检测和区分人类免疫缺陷病毒（HIV-1）。使用掠角沉积技术制造的高度优化的银纳米棒阵列用作SERS衬底。不同浓度（10 ²至10 ⁶在5种不同的HIV-1亚型（A，B，C，D和CRF02_AG）中鉴定出1个拷贝数/毫升）。显示了病毒在不使用抗体或中间试剂的情况下直接与Ag纳米棒的结合。将纯化的病毒掺入水中和健康血浆中以捕获纯HIV-1峰。还捕获了X4和R5热带菌株的不同峰，表明基于嗜性的检测。使用多变量工具进一步确认并统计分析了以上数据。因此，本研究表明SERS平台检测和区分HIV-1病毒的能力暗示了其使用临床标本和分离株的进一步验证。