This work was aimed at examining the protective effect of pinocembrin and pinostrobin in retinal pigment epithelial cells exposed to oxidative stress caused by hydrogen peroxide (H₂O₂). In the present study, the ARPE-19 cell line, one of the retinal pigment epithelial cell lines, was selected as the test cell culture. The incubation was performed on the basis of a 16-h application time of 500 μMH₂O₂ to induce the oxidative stress damage in the ARPE-19 cell line. The protective effect of applying pinocembrin and pinostrobin in H₂O₂-induced oxidative damage was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The obtained results showed evidence of therapeutic effects of pinocembrin and pinostrobin in cells exposed to oxidative stress-induced damage. Pinocembrin and pinostrobin can find direct medical application as pharmaceutical agents and may contribute to the design, synthesis and semi-synthesis of new drugs and to the development of chemical templates for treatment of diseases.

这项工作旨在检查pinocembrin和pinostrobin在暴露于过氧化氢（H ₂ O ₂）引起的氧化应激的视网膜色素上皮细胞中的保护作用。在本研究中，选择ARPE-19细胞系（一种视网膜色素上皮细胞系）作为测试细胞培养物。温育后的500μMH的16-H施加时间的基础上进行₂ Ò ₂以诱导ARPE-19细胞系中的氧化应激损伤。松树皮素和松果糖苷在H ₂ O _2中的保护作用MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化铵）测定法评估了诱导的氧化损伤。所获得的结果证明了松球蛋白和松果糖蛋白在暴露于氧化应激诱导的损伤的细胞中具有治疗作用。Pinocembrin和pinostrobin可以作为药物直接用于医学领域，并可能有助于新药的设计，合成和半合成以及用于治疗疾病的化学模板的开发。