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Investigation into the strain typing of Potato Virus Y isolates in Egypt
Journal of Plant Diseases and Protection ( IF 2 ) Pub Date : 2020-11-19 , DOI: 10.1007/s41348-020-00399-7
Esraa A. Elwan , Mona Rabie , Engy E. Abdel Aleem , Radwa M. Taha , Faiza A. Fattouh

Potato Virus Y (PVY) is well recognized world-wide as an important virus of potato with damaging effect on both the crop yield and quality. Recent research has revealed a wide range of PVY strain diversity and complexity. The high degree of PVY recombinant types reported in some areas, has intrigued investigation into the efficacy of tools used for strain identification. PCR and ELISA are routinely and reliably used for PVY detection, however, the degree of information reflecting strain genotypes by various identification tools is variable and incoherent. Overall incidence of PVY from different potato samples collected in 2018/2019 in some locations in Egypt is estimated by RT-PCR to be 12%. The most prevalent strain detected was of PVYN origin. Phylogeny analysis revealed high degree of relatedness to isolates from specific locations, which may indicate probable origin of the isolates. Identification of recombinant strains of PVYN origin by both M-RT-PCR and ELISA has indicated that PVYNTN, PVYN−Wilga and PVYN:O are the most prevalent recombinant types.



中文翻译:

埃及马铃薯Y病毒分离株的菌株类型调查

马铃薯病毒Y(PVY)在世界范围内被公认为一种重要的马铃薯病毒,会对作物的产量和品质造成破坏性影响。最近的研究揭示了广泛的PVY菌株多样性和复杂性。在某些地区报道的高度PVY重组类型引起了对用于菌株鉴定的工具效力的研究。PCR和ELISA常规可靠地用于PVY检测,但是,通过各种识别工具反映菌株基因型的信息程度是可变的且不一致。通过RT-PCR估计,2018/2019年埃及一些地区从不同马铃薯样品中收集到的PVY的总体发生率为12%。检测到的最普遍的应变是PVY N起源。系统发育分析表明与特定位置的分离株高度相关,这可能表明分离株可能起源。通过M-RT-PCR和ELISA鉴定PVY N来源的重组菌株表明,PVY NTN,PVY N-Wilga和PVY N:O是最流行的重组类型。

更新日期:2020-11-19
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