Abstract

In the current study, we have synthesized and characterized two, hitherto unreported, bis (N,N) donor dioxovanadium(V) complexes based on 1,10-phenanthroline (complex 1) and 2,2′-bipyridyl (complex 2) ligands with 3,5-dinitrosalicylic acid counter anion. The structure of the complexes are confirmed by single crystal X-ray crystallography. The binding interactions of the metal complexes with deoxyribonucleic acid (DNA) and bovine serum albumin (BSA) have been investigated by UV absorption titration and tryptophan emission quenching methods respectively. The complexes quench the intrinsic fluorescence of BSA with increasing amount of the complex concentration. Furthermore, the cytotoxic effect of the complexes against cervical cancer cell lines (HeLa) has been ascertained by MTT assay. The complexes are found to show good DNA/BSA binding ability. Based on the results obtained, it has been observed that the complexes bind preferably to groove as implicated by hyperchromic effect with increasing amount of the DNA. The binding constants (K_b) of the complexes 1 and 2 are 9.02 × 10³ M⁻¹ and 2.13 × 10³ M⁻¹ respectively. The quenching constants (K_BSA) and quenching rate constant (K_q) of the complexes 1 & 2 are 0.1075 × 10⁶ M⁻¹ & 0.076 × 10⁶ M⁻¹ and 1.075 × 10¹³ M⁻¹S⁻¹ and 0.76 × 10¹³ M⁻¹S⁻¹, respectively.

Graphic Abstract

中文翻译：

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偶然性二氧杂钒酸盐的合成，表征及其DNA / BSA相互作用研究和体外细胞毒性活性

摘要

在当前的研究中，我们已经合成和表征了两个迄今未报道的bis（N，N）基于1,10-菲咯啉（配合物1）和2,2'-联吡啶基（配合物2）配体与3,5-二硝基水杨酸抗衡阴离子的供体二氧钒钒（V）配合物。配合物的结构通过单晶X射线晶体学确认。分别通过紫外吸收滴定法和色氨酸发射猝灭法研究了金属配合物与脱氧核糖核酸（DNA）和牛血清白蛋白（BSA）的结合相互作用。随着配合物浓度的增加，配合物淬灭BSA的固有荧光。此外，已经通过MTT分析确定了复合物对宫颈癌细胞系（HeLa）的细胞毒性作用。发现该复合物显示出良好的DNA / BSA结合能力。根据获得的结果，业已发现，随着DNA量的增加，复合物优选结合到凹槽上，这与增色作用有关。结合常数（K配合物1和2的_b）分别为9.02×10 ³  M ^-1和2.13×10 ³  M ^-1。配合物1和2的猝灭常数（K _BSA）和猝灭速率常数（K _q）分别为0.1075×10 ⁶  M ^-1和0.076×10 ⁶  M ^-1和1.075×10 ¹³  M ^-1 S ^-1和0.76 ×10 ¹³  M ^-1 S ^-1。

图形摘要