In the present study, Rhus vernicifera laccase (RvLac) was immobilized through covalent methods on the magnetic nanoparticles. Fe₂O₃ and Fe₃O₄ nanoparticles activated by 3-aminopropyltriethoxysilane followed with glutaraldehyde showed maximum immobilization yields and relative activity up to 81.4 and 84.3% at optimum incubation and pH of 18 h and 5.8, respectively. The maximum RvLac loading of 156 mg/g of support was recorded on Fe₂O₃ nanoparticles. A higher optimum pH and temperature of 4.0 and 45 °C were noted for immobilized enzyme compared to values of 3.5 and 40 °C for free form, respectively. Immobilized RvLac exhibited better relative activity profiles at various pH and temperature ranges. The immobilized enzyme showed up to 16-fold improvement in the thermal stability, when incubated at 60 °C, and retained up to 82.9% of residual activity after ten cycles of reuses. Immobilized RvLac exhibited up to 1.9-fold higher bisphenol A degradation efficiency potential over free enzyme. Previous reports have demonstrated the immobilization of RvLac on non-magnetic supports. This study has demonstrated that immobilization of RvLac on magnetic nanoparticles is very efficient especially for achieving high loading, better pH and temperature profiles, and thermal- and solvents-stability, high reusability, and higher degradation of bisphenol A.

在本研究中，漆树漆酶（Rv Lac）通过共价方法固定在磁性纳米粒子上。由 3-氨基丙基三乙氧基硅烷和戊二醛活化的Fe ₂ O ₃和 Fe ₃ O ₄纳米颗粒在最佳温育和 18 小时和 5.8 的 pH 值下分别显示出最大的固定产率和高达 81.4% 和 84.3% 的相对活性。_{在 Fe 2} O ₃上记录的最大Rv Lac 负载为 156 mg/g 载体纳米粒子。与游离形式的 3.5 和 40 °C 的值相比，固定化酶的最佳 pH 值和温度分别为 4.0 和 45 °C。固定化的Rv Lac 在不同的 pH 值和温度范围内表现出更好的相对活性曲线。当在 60 °C 下孵育时，固定化酶的热稳定性提高了 16 倍，并且在重复使用 10 次后保留了高达 82.9% 的残余活性。固定化Rv Lac 的双酚 A 降解效率潜力比游离酶高 1.9 倍。以前的报告已经证明了Rv Lac 固定在非磁性载体上。本研究表明，Rv的固定化Lac on磁性纳米粒子非常有效，特别是对于实现高负载、更好的pH和温度曲线、热稳定性和溶剂稳定性、高重复使用性以及双酚A的更高降解性。