In cancer cells, some of the transporters are overexpressed. Therefore targeted contrast agent delivery by functionalized nanoparticles is probable to advanced molecular imaging and cancer cell identification in the early stages of cancer. In this research, folic acid-modified mesoporous silica nanospheres, loaded with gadolinium, were prepared and characterized by various techniques. FSG nanoprobe cellular uptake was analyzed quantitatively and qualitatively by ICP-AES, flow cytometry, and fluorescent microscopy in cell lines which expressed different levels of folic acid receptors. The cellular uptake of FSG nanoprobe by cancer cells was about 62% which is 2.6 times higher than cellular uptake of nanoprobe without folic acid targeting group. The average of FSG nanoprobe cellular uptake was 0.60.1 pg Gd³⁺ per cancer cell without any toxicity. The r ₁ relaxivity of this nanoprobe in cancer cells was measured at 10.16.0 mM⁻¹ s⁻¹ per Gd³⁺ base. The results demonstrated that the present synthesized nanoprobe is promising one for cancer cell detection and delivering a sufficient amount of Gd³⁺ as contrast agents into cancer cells.

在癌细胞中，某些转运蛋白过表达。因此，在癌症的早期阶段，通过功能化的纳米粒子靶向地输送造影剂很可能有助于先进的分子成像和癌细胞鉴定。在这项研究中，用various制备了叶酸修饰的介孔二氧化硅纳米球，并通过各种技术对其进行了表征。在表达不同水平叶酸受体的细胞系中，通过ICP-AES，流式细胞仪和荧光显微镜对FSG纳米探针的细胞摄取进行了定量和定性分析。癌细胞对FSG纳米探针的细胞吸收率约为62％，是没有叶酸靶向基团的纳米探针对细胞吸收率的2.6倍。FSG纳米探针细胞摄取的平均值为0.60.1 pg Gd ³⁺每个癌细胞没有任何毒性。该纳米探针在癌细胞中的r ₁弛豫度测量为每Gd ³⁺碱为10.16.0 mM ^-1 s ^-1。结果表明，本发明的合成纳米探针有望用于癌细胞检测并将足够量的Gd ³⁺作为造影剂递送至癌细胞中。