Influence of interference effects on the spectral quality and histological classification by FT-IR imaging in transflection geometry,Analyst

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Influence of interference effects on the spectral quality and histological classification by FT-IR imaging in transflection geometry
Analyst ( IF 3.6 ) Pub Date : 2020-11-9 , DOI: 10.1039/d0an01565b
Danuta Liberda _{1,

2,

3,

4,

5} , Paulina Koziol _{1,

2,

3,

4,

5} , Magda K. Raczkowska _{4,

5,

6,

7,

8} , Wojciech M. Kwiatek _{4,

5,

6} , Tomasz P. Wrobel _{1,

2,

3,

4,

5}

Affiliation

Infrared (IR) imaging can be used for fast, accurate and non-destructive pathology recognition of biopsies when supported by machine learning algorithms. Transflection mode of measurements has the potential to be translated into the clinic due to economic reasons of large-scale imaging with the need for inexpensive substrates. Unfortunately, in this mode spectral distortions originating from light interference appear. Due to this fact transmission measurement mode is more frequently used in pathology recognition. Nevertheless, this measurement mode also is not devoid of spectral distortion effects like scattering. However, this effect is better understood and there are preprocessing algorithms to minimize it. In this work, we investigated the influence of interference effects on spectral quality of pancreatic tissues measured in transmission and transflection mode with Fourier tranform IR (FT-IR) microscopy using samples embedded with and without paraffin. The removal of paraffin leads to an altered magnitude of interference in transflection and provides a platform for a detailed analysis of its effect on the spectra of biological material, since the same sample is measured with different interference conditions. Moreover, the potential of transflection mode measurements in histological classification of analyzed samples was investigated and compared with classification results for transmission mode.

中文翻译：

反射几何中干涉效应对FT-IR成像光谱质量和组织学分类的影响

当机器学习算法支持时，红外（IR）成像可用于快速，准确和无损的活检病理学识别。由于需要廉价的基板进行大规模成像的经济原因，测量的透射模式有可能转化为临床。不幸的是，在这种模式下，会出现源自光干扰的光谱失真。由于这个事实，透射测量模式在病理学识别中被更频繁地使用。然而，这种测量模式也没有没有像散射这样的光谱失真效应。但是，可以更好地理解这种效果，并且有一些预处理算法可以将其最小化。在这项工作中我们调查了傅里叶变换红外（FT-IR）显微镜使用嵌入和不嵌入石蜡的样品在透射和透射模式下测量的干扰对胰腺组织光谱质量的影响。石蜡的去除会导致透射反射中干扰强度的改变，并为详细分析其对生物材料光谱的影响提供了平台，因为同一样品是在不同的干扰条件下测量的。此外，研究了透射模式测量在分析样品的组织学分类中的潜力，并将其与透射模式的分类结果进行了比较。石蜡的去除会导致透射反射中干扰强度的改变，并为详细分析其对生物材料光谱的影响提供了平台，因为同一样品是在不同的干扰条件下测量的。此外，研究了透射模式测量在分析样品的组织学分类中的潜力，并将其与透射模式的分类结果进行了比较。石蜡的去除会导致透射反射中干扰强度的改变，并为详细分析其对生物材料光谱的影响提供了平台，因为同一样品是在不同的干扰条件下测量的。此外，研究了透射模式测量在分析样品的组织学分类中的潜力，并将其与透射模式的分类结果进行了比较。

更新日期：2020-12-09

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