In this study, we firstly investigated the surface and protein adsorption properties of montmorillonite (MMT)/chitosan (CS) composite films with various MMT/CS weight ratios for metallic implants coating applications. Bicinchoninic acid (BCA) protein assay results show that the neat CS film exhibits a high concentration of bovine serum albumin (BSA) protein adhesion because the abundant carbonyl and amide functional groups on the surface of the CS film easily form hydrogen bonds with the copious carboxylic acid groups on the surface of the BSA protein. The MMT/CS composite films with MMT/CS = 3, 5, 8, and 10 possess a much lower BSA adhesion concentration than that of the neat CS film, as some of the carbonyl and amide functional groups on the surface of the composite films are replaced by the –Si–O–Si and –Al–O–Al groups. Among these MMT/CS composite films, the film with MMT/CS = 5 exhibits the lowest BSA adsorption concentration because it possesses a higher MMT content than those with MMT/CS = 1 and 3 and a smoother and non-porous surface than those with MMT/CS = 8 and 10. According to our results, MMT/CS composite films with appropriate MMT/CS weight ratios exhibit better surface and protein adsorption properties than neat CS for biomedical applications.

在这项研究中，我们首先研究了具有不同MMT / CS重量比的蒙脱土（MMT）/壳聚糖（CS）复合膜的表面和蛋白质吸附性能，适用于金属植入物涂层应用。双辛可宁酸（BCA）蛋白测定结果表明，纯净的CS膜表现出高浓度的牛血清白蛋白（BSA）蛋白粘附力，因为CS膜表面上丰富的羰基和酰胺官能团容易与大量羧基形成氢键BSA蛋白表面的酸性基团。MMT / CS = 3、5、8和10的MMT / CS复合膜具有比纯CS膜低得多的BSA附着浓度，因为复合膜表面上的一些羰基和酰胺官能团被–Si–O–Si和–Al–O–Al组取代。