We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.

中文翻译：

---

双视角斜面显微镜（dOPM）

我们提出了一种称为dOPM的新型折叠式双视角斜面显微镜（OPM）技术，该技术通过在重新聚焦空间中平移一对倾斜的反射镜来获得样品的两个正交视图。使用40倍1.15 NA的水浸主物镜，测量的200纳米珠的去卷积图像体积的半峰全宽（FWHM）横向为0.35±0.04 µm，轴向为0.39±0.02 µm，轴向为0.81±0.07 µm。使用光片FWHM在4.99±0.58 µm和4.89±0.63 µm的两个视图的框架中测得的z截面值是1.33±0.45 µm。为了定性地证明该系统可以减少阴影伪影，同时提供更高的各向同性分辨率，对直径约100 µm的多细胞球体成像。