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Measurement of Alcohol-Dependent Physiological Changes in Red Blood Cells Using Resistive Pulse Sensing
ACS Sensors ( IF 8.2 ) Pub Date : 2020-11-18 , DOI: 10.1021/acssensors.0c01302
Saurabh Kaushik 1 , Manohara Mahadeva 1 , Kandhasamy Durai Murugan 1 , Varadharajan Sundaramurthy 2 , Gautam Vivek Soni 1
Affiliation  

Alcohol exposure has been postulated to adversely affect the physiology and function of the red blood cells (RBCs). The global pervasiveness of alcohol abuse, causing health issues and social problems, makes it imperative to resolve the physiological effects of alcohol on RBC physiology. Alcohol consumed recreationally or otherwise almost immediately alters cell physiology in ways that is subtle and still unresolved. In this paper, we introduce a high-resolution device for quantitative electrofluidic measurement of changes in RBC volume upon alcohol exposure. We present an exhaustive calibration of our device using model cells to measure and resolve volume changes down to 0.6 fL. We find an RBC shrinkage of 5.3% at 0.125% ethanol (the legal limit in the United States) and a shrinkage of 18.5% at 0.5% ethanol (the lethal limit) exposure. Further, we also measure the time dependence of cell volume shrinkage (upon alcohol exposure) and then recovery (upon alcohol removal) to quantify shrinkage and recovery of RBC volumes. This work presents the first direct quantification of temporal and concentration-dependent changes in red blood cell volume upon ethanol exposure. Our device presents a universally applicable high-resolution and high-throughput platform to measure changes in cell physiology under native and diseased conditions.

中文翻译:

使用电阻脉冲传感测量红细胞中酒精依赖的生理变化

假定饮酒会对红细胞(RBC)的生理和功能产生不利影响。酒精滥用在全球范围内普遍存在,导致健康问题和社会问题,因此必须解决酒精对RBC生理的生理影响。酒精消遣性地或以其他方式几乎立即以细微且仍未解决的方式改变细胞生理。在本文中,我们介绍了一种高分辨率的设备,用于定量电醇测量酒精暴露后红细胞体积的变化。我们使用模型池对设备进行详尽的校准,以测量和解决体积变化低至0.6 fL的情况。我们发现,在0.125%乙醇(美国的法定限量)下,RBC收缩为5.3%,在0.5%乙醇(致命极限)下,RBC收缩为18.5%。进一步,我们还测量了细胞体积收缩(依赖酒精暴露)然后恢复(依赖酒精去除)随时间的变化,以量化收缩和RBC体积恢复。这项工作提出了乙醇暴露后红细胞体积中时间和浓度依赖性变化的首次直接定量分析。我们的设备提供了一种通用的高分辨率和高通量平台,可以测量自然和患病条件下细胞生理学的变化。
更新日期:2020-12-24
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