Abstract

Diets rich in fats are linked to elevated systemic inflammation, which augments the progression of inflammatory-related disorders including non-alcoholic fatty liver disease (NAFLD) and neurodegenerative diseases. A phenolic-enriched pomegranate fruit extract (PE) was investigated for its hepatoprotective and anti-inflammatory effects in male C57BL/6 mice fed either a high-fat diet or a standard rodent diet with or without 1% of PE for 12 weeks. Mouse livers and hippocampi were evaluated for the expression of genes associated with NAFLD and inflammation by multiplexed gene analysis. PE alleviated diet-induced fatty liver and suppressed hepatic lipid regulating genes including Cd36, Fas, Acot2 and Slc27a1. In addition, PE suppressed gene expression of pro-inflammatory cytokines including Il-1α, Il-7, Il-11, Ifnα, Tnfα and Lepr in the hippocampi. Our findings support the protective effects of PE against high-fat diet-induced hepatic and neurological disease.

摘要

富含脂肪的饮食与全身炎症升高有关，这会加剧炎症相关疾病的进展，包括非酒精性脂肪肝（NAFLD）和神经退行性疾病。研究了富含酚类的石榴果实提取物 (PE) 对雄性 C57BL/6 小鼠的保肝和抗炎作用，该小鼠喂食高脂肪饮食或标准啮齿动物饮食（含或不含 1% PE）持续 12 周。通过多重基因分析评估小鼠肝脏和海马与 NAFLD 和炎症相关基因的表达。PE减轻饮食诱导的脂肪肝并抑制包括Cd36、Fas、Acot2和Slc27a1在内的肝脏脂质调节基因. 此外，PE抑制海马中促炎细胞因子的基因表达，包括Il-1α、Il-7、Il-11、Ifnα、Tnfα和Lepr。我们的研究结果支持 PE 对高脂饮食引起的肝脏和神经系统疾病的保护作用。