Abstract

Sorghum is a globally important C4 crop that serves both as staple food and as bio-fuel, and showed high phenotypic variation in grain quality. In this study, we performed RNA-seq in grains of three local Sorghum cultivars Z6, Z27 and HC4, which have differential agronomic traits especially in grain color. Our analysis revealed more than 3000 differentially expressed genes among the three cultivars. The differentially expressed genes were enriched in carbohydrate, amino acid and flavonoid metabolic pathways, which may affect the appearance and nutrition value of grains. We also identified new genes and SNPs in the three cultivars. Our results revealed the transcriptome variations among different Sorghum cultivars that correlates with grain nutrition, and provided valuable information for sorghum breeding.

摘要

高粱是一种全球重要的 C4 作物，既可作为主食，又可作为生物燃料，并且在谷物质量方面表现出高度的表型变异。在这项研究中，我们对三种当地高粱品种 Z6、Z27 和 HC4的籽粒进行了 RNA-seq，这些品种具有不同的农艺性状，特别是在籽粒颜色方面。我们的分析揭示了三个品种中超过 3000 个差异表达的基因。差异表达基因在碳水化合物、氨基酸和类黄酮代谢途径中富集，可能影响谷物的外观和营养价值。我们还鉴定了三个品种中的新基因和 SNP。我们的结果揭示了不同高粱之间的转录组变异与谷物营养相关的品种，为高粱育种提供了有价值的信息。