A rapid detection of Escherichia coli O157:H7 by competition visual antigen macroarray,Journal of Food Safety

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A rapid detection of Escherichia coli O157:H7 by competition visual antigen macroarray
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-11-17 , DOI: 10.1111/jfs.12872
Junfang Xia _{1,

2} , Shi Qiu ₁ , Haijuan Zeng ₁ , Cheng Liu ₁ , Qing Liu ₁

Affiliation

Pathogenic bacterial contamination is a serious problem for the food industry and in public health. Rapid, accurate and affordable testing for pathogenic bacterial strains is desirable. In this study, a competition visual antigen macroarray (CVAM) for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) has been developed. This array was able to utilize an HRP‐labeled anti‐E. coli O157:H7 MAb at a concentration of 1:20000 while having a similar sensitivity of 10 ⁵ CFU/ml for E. coli O157:H7 detection as double antibody sandwich array and conventional ELISA. The detection time of CVAM was short as 2 h and can be examined directly by the naked eye. Moreover, there was no cross‐reactivity in the detection of tested in this study. Application of CVAM for the detection of E. coli O157:H7 artificially contaminated milk samples was feasible that showed the antigen array could be applied in the detection of food pathogen infections.

中文翻译：

竞争性视觉抗原大阵列快速检测大肠杆菌O157：H7

致病细菌污染对于食品工业和公共卫生是一个严重的问题。需要对病原细菌菌株进行快速，准确和负担得起的测试。在这项研究中，已经开发出一种竞争性视觉抗原大阵列（CVAM），用于快速检测大肠杆菌O157：H7（E. coli O157：H7）。该阵列能够使用浓度为1：20000的HRP标记的抗大肠杆菌O157：H7 MAb，同时对大肠杆菌的敏感性为10 ⁵ CFU / mlO157：H7检测为双抗体夹心阵列和常规ELISA。CVAM的检测时间短至2 h，可以用肉眼直接检查。此外，在这项研究中检测到的检测没有交叉反应。将CVAM用于检测大肠杆菌O157：H7人为污染的牛奶样品是可行的，表明该抗原阵列可用于检测食品病原体感染。

更新日期：2020-11-17

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