Multi‐approach comparative study of the two most prevalent genotypes of pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) in Chile,Entomological Science

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Multi‐approach comparative study of the two most prevalent genotypes of pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) in Chile
Entomological Science ( IF 0.7 ) Pub Date : 2020-11-17 , DOI: 10.1111/ens.12436
Laurent Serteyn ₁ , Emilie Bosquee ₁ , Anaïs Delatour ₁ , Maria E. Rubio‐Melendez ₂ , Karla V. Vega‐Muñoz ₂ , Claudio C. Ramirez ₂ , Frédéric Francis ₁

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The pea aphid, Acyrthosiphon pisum Harris, is able to colonize various habitats and form genetically distinct biotypes worldwide. In Chile, few genotypes are dominating in space and time and are qualified as “superclones”. Here, we compared in the laboratory different biological features of the most common clones of A. pisum collected from pea (Ap1) and alfalfa (Ap2) fields, in order to gain some insight on their differential ability to colonize broad bean plants. We also studied their probing behavior, profiled their proteome and characterized their community of endosymbionts. We found that the Ap1 genotype performed better on Vicia faba and realized more salivation events in phloem than Ap2. In addition, Ap1 presented a higher prevalence of the endosymbiont Serratia symbiotica. Among the total of 40 proteins that were differentially expressed, 14 and 26 were upregulated in Ap1 and Ap2 genotypes, respectively. The symbionin from Buchnera aphidicola was found to be upregulated in Ap1. A field experiment showed that both genotypes were able to colonize wild legumes, with Ap1 reproducing better on Vicia nigricans than Ap2. However, Ap2 exhibited higher reproduction in the other three wild legumes, suggesting higher invasiveness capacity on wild plants. Variation in the ability to colonize, feeding behavior and the putative involvement of differentially regulated proteins between Ap1 and Ap2 are discussed in relation to their respective endosymbiotic composition, nutritional lifestyle and consequences on their “superclone” status.

中文翻译：

智利豌豆蚜虫最主要的两种基因型的多方法比较研究（Hemiptera：Aphididae）

豌豆蚜虫（Acyrthosiphon pisum Harris）能够在各种栖息地定居，并在全球范围内形成遗传上不同的生物型。在智利，很少有基因型在时空上占主导地位，被称为“超级克隆”。在这里，我们最常见的克隆实验室不同的生物学特性比较A.豌豆豌豆（API）和紫花苜蓿（AP2）领域收集，以获得他们的差殖民蚕豆植株能力的一些见解。我们还研究了他们的探测行为，分析了他们的蛋白质组，并对他们的共生共生体进行了表征。我们发现Ap1基因型在蚕豆上表现更好并在韧皮部实现了比Ap2更多的流涎事件。此外，Ap1呈现较高的共生内生沙雷氏菌共生菌。在总共40种差异表达的蛋白中，分别有14种和26种在Ap1和Ap2基因型中上调。发现来自蚜虫双歧杆菌的共生素在Ap1中被上调。田间试验表明，两种基因型都能在野生豆科植物上定殖，Ap1在野豌豆上繁殖更好比Ap2 然而，Ap2在其他三种野生豆科植物中表现出更高的繁殖能力，表明对野生植物具有更高的入侵能力。讨论了Ap1和Ap2之间的定居能力，进食行为和差异调节蛋白的假定参与方面的变化，涉及它们各自的内共生成分，营养生活方式以及对其“超级克隆”状态的影响。

更新日期：2020-11-17

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