Abscisic acid receptors maintain abscisic acid homeostasis by modulating UGT71C5 glycosylation activity,Journal of Integrative Plant Biology

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Abscisic acid receptors maintain abscisic acid homeostasis by modulating UGT71C5 glycosylation activity
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-11-18 , DOI: 10.1111/jipb.13030
Yanlin Ma ₁ , Jing Cao ₁ , Qiaoqiao Chen ₁ , Jiahan He ₁ , Zhibin Liu ₁ , Jianmei Wang ₁ , Xufeng Li ₁ , Yi Yang ₁

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Uridine diphosphate‐glucosyltransferases (UGTs) maintain abscisic acid (ABA) homeostasis in Arabidopsis thaliana by converting ABA to abscisic acid‐glucose ester (ABA‐GE). UGT71C5 plays an important role in the generation of ABA‐GE. Abscisic acid receptors are crucial upstream components of the ABA signaling pathway, but how UGTs and ABA receptors function together to modulate ABA levels is unknown. Here, we demonstrated that the ABA receptors RCAR12/13 and UGT71C5 maintain ABA homeostasis in Arabidopsis following rehydration under drought stress. Biochemical analyses show that UGT71C5 directly interacted with RCAR8/12/13 in yeast cells, and the interactions between UGT71C5 and RCAR12/13 were enhanced by ABA treatment. Enzyme activity analysis showed that ABA‐GE contents were significantly elevated in the presence of RCAR12 or RCAR13, suggesting that these ABA receptors enhance the activity of UGT71C5. Determination of the content of ABA and ABA‐GE in Arabidopsis following rehydration under drought stress revealed that ABA‐GE contents were significantly higher in Arabidopsis plants overexpressing RCAR12 and RCAR13 than in non‐transformed plants and plants overexpressing RCAR11 following rehydration under drought stress. These observations suggest that RCAR12 and RCAR13 enhance the activity of UGT71C5 to glycosylate excess ABA into ABA‐GE following rehydration under drought stress, representing a rapid mechanism for regulating plant growth and development.

中文翻译：

脱落酸受体通过调节UGT71C5糖基化活性维持脱落酸稳态

尿苷二磷酸-葡萄糖基转移酶（UGTs）通过将ABA转化为脱落酸-葡萄糖酯（ABA-GE）来维持拟南芥中的脱落酸（ABA）稳态。UGT71C5在ABA‐GE的产生中起着重要作用。脱落酸受体是ABA信号传导途径的关键上游成分，但是UGT和ABA受体如何共同发挥作用来调节ABA水平尚不清楚。在这里，我们证明了ABA受体RCAR12 / 13和UGT71C5维持拟南芥中的ABA稳态。在干旱胁迫下补液。生化分析表明，UGT71C5与酵母细胞中的RCAR8 / 12/13直接相互作用，而ABA处理增强了UGT71C5与RCAR12 / 13之间的相互作用。酶活性分析表明，在存在RCAR12或RCAR13的情况下，ABA-GE含量显着升高，表明这些ABA受体增强了UGT71C5的活性。ABA和ABA-GE在含量测定拟南芥以下干旱胁迫下补液透露，ABA-GE含量均高于显著拟南芥植株过表达RCAR12和RCAR13比非转基因植物和植物过度RCAR11在干旱胁迫下补液。这些观察结果表明，RCAR12和RCAR13增强了UGT71C5的活性，使其在干旱胁迫下复水后将过量的ABA糖基化为ABA-GE，代表了调节植物生长和发育的快速机制。

更新日期：2020-11-18

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