当前位置：
X-MOL 学术
›
Microbiologyopen
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-11-18 , DOI: 10.1002/mbo3.1131 Lan Zhang 1 , Caiwu Li 2 , Yaru Zhai 1 , Lan Feng 1 , Keke Bai 1 , Zhizhong Zhang 2 , Yan Huang 2 , Ti Li 2 , Desheng Li 2 , Hao Li 1 , Pengfei Cui 1 , Danyu Chen 1 , Hongning Wang 1 , Xin Yang 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-11-18 , DOI: 10.1002/mbo3.1131 Lan Zhang 1 , Caiwu Li 2 , Yaru Zhai 1 , Lan Feng 1 , Keke Bai 1 , Zhizhong Zhang 2 , Yan Huang 2 , Ti Li 2 , Desheng Li 2 , Hao Li 1 , Pengfei Cui 1 , Danyu Chen 1 , Hongning Wang 1 , Xin Yang 1
Affiliation
|
In this study, a total of 14 vaginal samples (GPV1‐14) from giant pandas were analyzed. These vaginal samples were divided into two groups as per the region and age of giant pandas. All the vaginal samples were analyzed using metagenomic sequencing. As per the outcomes of metagenomic analysis, Proteobacteria (39.04%), Firmicutes (5.27%), Actinobacteria (2.94%), and Basidiomycota (2.77%) were found to be the dominant phyla in the microbiome of the vaginal samples. At the genus level, Pseudomonas (21.90%) was found to be the most dominant genus, followed by Streptococcus (3.47%), Psychrobacter (1.89%), and Proteus (1.38%). Metastats analysis of the microbial species in the vaginal samples of giant pandas from Wolong Nature Reserve, Dujiangyan and Ningbo Youngor Zoo, and Ya'an Bifengxia Nature Reserve was found to be significantly different (p < 0.05). Age groups, that is, AGE1 (5‐10 years old) and AGE2 (11‐16 years old), also demonstrated significantly different inter‐group microbial species (p < 0.05). For the first time, Chlamydia and Neisseria gonorrhoeae were detected in giant pandas’ reproductive tract. GPV3 vaginal sample (2.63%) showed highest Chlamydia content followed by GPV14 (0.91%), and GPV7 (0.62%). GPV5 vaginal sample (7.17%) showed the highest Neisseria gonorrhoeae content, followed by GPV14 (7.02%), and GPV8 (6.50%). Furthermore, we employed eggNOG, CAZy, KEGG, and NCBI databases to investigate the functional significance of giant panda's vaginal microbial community. The outcomes indicated that giant panda's vaginal microbes were involved in biological processes. The data from this study will help in improving the reproductive health of giant pandas.
中文翻译：
利用宏基因组学测序分析大熊猫阴道微生物组
在这项研究中，共分析了来自大熊猫的 14 个阴道样本（GPV1-14）。这些阴道样本根据大熊猫的地区和年龄分为两组。使用宏基因组测序分析所有阴道样品。根据宏基因组分析的结果，发现变形菌门 (39.04%)、厚壁菌门 (5.27%)、放线菌门 (2.94%) 和担子菌门 (2.77%) 是阴道样本微生物组中的优势门。在属水平上，假单胞菌(21.90%) 被发现是最主要的属，其次是链球菌(3.47%)、假单胞菌(1.89%) 和变形杆菌(1.38%)。卧龙自然保护区、都江堰、宁波雅戈尔动物园和雅安碧峰峡自然保护区大熊猫阴道样本微生物种类的Metastats分析发现差异显着（p < 0.05）。年龄组，即 AGE1（5-10 岁）和 AGE2（11-16 岁）也表现出显着不同的组间微生物种类（p < 0.05）。首次 在大熊猫生殖道中检测到衣原体 和 淋病奈瑟菌。GPV3 阴道样本 (2.63%) 显示最高的衣原体含量，其次是 GPV14 (0.91%) 和 GPV7 (0.62%)。GPV5 阴道样本 (7.17%) 显示淋病奈瑟菌最高含量，其次是 GPV14 (7.02%) 和 GPV8 (6.50%)。此外，我们利用eggNOG、CAZy、KEGG和NCBI数据库来研究大熊猫阴道微生物群落的功能意义。结果表明，大熊猫的阴道微生物参与了生物过程。这项研究的数据将有助于改善大熊猫的生殖健康。
更新日期：2020-12-23
中文翻译：
利用宏基因组学测序分析大熊猫阴道微生物组
在这项研究中，共分析了来自大熊猫的 14 个阴道样本（GPV1-14）。这些阴道样本根据大熊猫的地区和年龄分为两组。使用宏基因组测序分析所有阴道样品。根据宏基因组分析的结果，发现变形菌门 (39.04%)、厚壁菌门 (5.27%)、放线菌门 (2.94%) 和担子菌门 (2.77%) 是阴道样本微生物组中的优势门。在属水平上，假单胞菌(21.90%) 被发现是最主要的属，其次是链球菌(3.47%)、假单胞菌(1.89%) 和变形杆菌(1.38%)。卧龙自然保护区、都江堰、宁波雅戈尔动物园和雅安碧峰峡自然保护区大熊猫阴道样本微生物种类的Metastats分析发现差异显着（p < 0.05）。年龄组，即 AGE1（5-10 岁）和 AGE2（11-16 岁）也表现出显着不同的组间微生物种类（p < 0.05）。首次 在大熊猫生殖道中检测到衣原体 和 淋病奈瑟菌。GPV3 阴道样本 (2.63%) 显示最高的衣原体含量，其次是 GPV14 (0.91%) 和 GPV7 (0.62%)。GPV5 阴道样本 (7.17%) 显示淋病奈瑟菌最高含量，其次是 GPV14 (7.02%) 和 GPV8 (6.50%)。此外，我们利用eggNOG、CAZy、KEGG和NCBI数据库来研究大熊猫阴道微生物群落的功能意义。结果表明，大熊猫的阴道微生物参与了生物过程。这项研究的数据将有助于改善大熊猫的生殖健康。
京公网安备 11010802027423号