DNA‐Origami‐Based Assembly of Au@Ag Nanostar Dimer Nanoantennas for Label‐Free Sensing of Pyocyanin,ChemPhysChem

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DNA‐Origami‐Based Assembly of Au@Ag Nanostar Dimer Nanoantennas for Label‐Free Sensing of Pyocyanin
ChemPhysChem ( IF 2.3 ) Pub Date : 2020-11-18 , DOI: 10.1002/cphc.202000805
Vishaldeep Kaur ₁ , Swati Tanwar ₁ , Gagandeep Kaur ₁ , Tapasi Sen ₁

Affiliation

Early‐stage detection of diseases caused by pathogens is a prerequisite for expedient patient care. Due to the limited signal‐to‐noise ratio, molecular diagnostics needs molecular signal amplification after recognition of the target molecule. In this present study, we demonstrate the design of plasmonically coupled bimetallic Ag coated Au nanostar dimers with controlled nanogap using rectangular DNA origami. We further report the utility of the designed nanostar dimer structures as efficient SERS substrate for the ultrasensitive and label‐free detection of the pyocyanin molecule, which is a biomarker of the opportunistic pathogenic bacteria, Pseudomonas aeruginosa. The experimental results showed that the detection limit of pyocyanin with such nanoantenna based biosensor was 335 pM, which is much lower than the clinical range of detection. Thus, fast, sensitive and label‐free detection of pyocyanin at ultralow concentration in an infected human body can pave a facile route for early stage warning for severe bacterial infections.

中文翻译：

基于DNA折纸的Au @ Ag Nanostar Dimer纳米天线组装体，用于无标记的氰化青绿感应。

对由病原体引起的疾病进行早期检测是方便患者护理的前提。由于信噪比有限，因此分子诊断需要在识别目标分子后放大分子信号。在本研究中，我们演示了使用矩形DNA折纸通过等离子隙控制的等离子体耦合双金属银涂层的Au纳米星二聚体的设计。我们进一步报告了设计的nanostarstar二聚体结构作为高效SERS底物的效用，用于超灵敏和无标记的检测绿脓素分子，这是机会病原细菌铜绿假单胞菌的生物标志物。实验结果表明，用这种基于纳米天线的生物传感器对绿脓素的检出限为335 pM，远低于临床检测范围。因此，在感染的人体内以超低浓度快速，灵敏且无标签地检测到绿脓素可以为早期的严重细菌感染预警提供便利的途径。

更新日期：2021-01-21

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