High‐Definition Single‐Cell Printing: Cell‐by‐Cell Fabrication of Biological Structures,Advanced Materials

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High‐Definition Single‐Cell Printing: Cell‐by‐Cell Fabrication of Biological Structures
Advanced Materials ( IF 27.4 ) Pub Date : 2020-11-18 , DOI: 10.1002/adma.202005346
Pengfei Zhang ₁ , Adam R Abate _{1,

2,

3}

Affiliation

Bioprinting is a powerful technology with the potential to transform medical device manufacturing, organ replacement, and the treatment of diseases and physiologic malformations. However, current bioprinters are unable to reliably print the fundamental unit of all living things, single cells. A high‐definition single‐cell printing, a novel microfluidic technology, is presented here that can accurately print single cells from a mixture of multiple candidates. The bioprinter employs a highly miniaturized microfluidic sorter to deterministically select single cells of interest for printing, achieving an accuracy of ≈10 µm and speed of ≈100 Hz. This approach is demonstrated by fabricating intricate cell patterns with pre‐defined features through selective single‐cell printing. The approach is used to synthesize well‐defined spheroids with controlled composition and morphology. The speed, accuracy, and flexibility of the approach will advance bioprinting to enable new studies in organoid science, tissue engineering, and spatially targeted cell therapies.

中文翻译：

高清单细胞打印：生物结构的逐个细胞制造

生物打印是一项强大的技术，有可能改变医疗设备制造、器官替代以及疾病和生理畸形的治疗。然而，当前的生物打印机无法可靠地打印所有生物的基本单位——单细胞。这里提出了一种高清单细胞打印，这是一种新颖的微流体技术，可以从多个候选者的混合物中准确地打印单细胞。该生物打印机采用高度小型化的微流体分选器来确定性地选择感兴趣的单细胞进行打印，实现约 10 µm 的精度和约 100 Hz 的速度。通过选择性单细胞打印制造具有预定义特征的复杂细胞图案来证明这种方法。该方法用于合成具有受控成分和形态的明确定义的球体。该方法的速度、准确性和灵活性将推动生物打印，从而使类器官科学、组织工程和空间靶向细胞疗法的新研究成为可能。

更新日期：2020-12-28

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