当前位置：
X-MOL 学术
›
Toxicol. Lett.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Comparison of the biological impact of aerosol of e-vapor device with MESH® technology and cigarette smoke on human bronchial and alveolar cultures
Toxicology Letters ( IF 2.9 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.toxlet.2020.11.006 Albert Giralt 1 , Anita R Iskandar 1 , Florian Martin 1 , Elisa Moschini 2 , Tomasso Serchi 2 , Athanasios Kondylis 1 , Diego Marescotti 1 , Patrice Leroy 1 , Laura Ortega-Torres 1 , Shoaib Majeed 1 , Celine Merg 1 , Keyur Trivedi 1 , Emmanuel Guedj 1 , Stefan Frentzel 1 , Nikolai V Ivanov 1 , Manuel C Peitsch 1 , Arno C Gutleb 2 , Julia Hoeng 1
Toxicology Letters ( IF 2.9 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.toxlet.2020.11.006 Albert Giralt 1 , Anita R Iskandar 1 , Florian Martin 1 , Elisa Moschini 2 , Tomasso Serchi 2 , Athanasios Kondylis 1 , Diego Marescotti 1 , Patrice Leroy 1 , Laura Ortega-Torres 1 , Shoaib Majeed 1 , Celine Merg 1 , Keyur Trivedi 1 , Emmanuel Guedj 1 , Stefan Frentzel 1 , Nikolai V Ivanov 1 , Manuel C Peitsch 1 , Arno C Gutleb 2 , Julia Hoeng 1
Affiliation
|
Exposure to aerosol from electronic vapor (e-vapor) products has been suggested to result in less risk of harm to smokers than cigarette smoke (CS) exposure. Although many studies on e-vapor products have tested the effects of liquid formulations on cell cultures, few have evaluated the effects of aerosolized formulations. We examined the effects of acute exposure to the aerosol of an e-vapor device that uses the MESH® technology (IQOS® MESH, Philip Morris International) and to CS from the 3R4F reference cigarette on human organotypic bronchial epithelial culture and alveolar triculture models. In contrast to 3R4F CS exposure, exposure to the IQOS MESH aerosol (Classic Tobacco flavor) did not cause cytotoxicity in bronchial epithelial cultures or alveolar tricultures despite its greater concentrations of deposited nicotine (3- and 4-fold, respectively). CS exposure caused a marked decrease in the frequency and active area of ciliary beating in bronchial cultures, whereas IQOS MESH aerosol exposure did not. Global mRNA expression and secreted protein profiles revealed a significantly lower impact of IQOS MESH aerosol exposure than 3R4F CS exposure. Overall, our whole aerosol exposure study shows a clearly reduced impact of IQOS MESH aerosol relative to CS in bronchial and alveolar cultures, even at greater nicotine doses.
中文翻译：
采用 MESH® 技术的电子烟装置的气溶胶与香烟烟雾对人体支气管和肺泡培养物的生物影响的比较
有人认为，与接触香烟烟雾 (CS) 相比，接触电子蒸气 (e-vapor) 产品的气溶胶对吸烟者造成的伤害风险更小。尽管许多关于电子蒸汽产品的研究已经测试了液体制剂对细胞培养物的影响，但很少评估雾化制剂的影响。我们研究了急性暴露于使用 MESH® 技术（IQOS® MESH，Philip Morris International）的电子蒸汽装置的气溶胶和 3R4F 参考香烟中的 CS 对人类器官型支气管上皮培养和肺泡三培养模型的影响。与 3R4F CS 暴露相比，暴露于 IQOS MESH 气雾剂（经典烟草口味）不会导致支气管上皮培养物或肺泡三培养物中的细胞毒性，尽管其沉积的尼古丁浓度更高（分别为 3 倍和 4 倍）。 CS暴露导致支气管培养物中纤毛跳动的频率和活跃面积显着减少，而IQOS MESH气溶胶暴露则没有。整体 mRNA 表达和分泌蛋白谱显示，IQOS MESH 气溶胶暴露的影响明显低于 3R4F CS 暴露的影响。总体而言，我们的整个气溶胶暴露研究表明，即使在更大的尼古丁剂量下，相对于 CS，IQOS MESH 气溶胶对支气管和肺泡培养物的影响也明显降低。
更新日期：2021-02-01
中文翻译：
采用 MESH® 技术的电子烟装置的气溶胶与香烟烟雾对人体支气管和肺泡培养物的生物影响的比较
有人认为，与接触香烟烟雾 (CS) 相比，接触电子蒸气 (e-vapor) 产品的气溶胶对吸烟者造成的伤害风险更小。尽管许多关于电子蒸汽产品的研究已经测试了液体制剂对细胞培养物的影响，但很少评估雾化制剂的影响。我们研究了急性暴露于使用 MESH® 技术（IQOS® MESH，Philip Morris International）的电子蒸汽装置的气溶胶和 3R4F 参考香烟中的 CS 对人类器官型支气管上皮培养和肺泡三培养模型的影响。与 3R4F CS 暴露相比，暴露于 IQOS MESH 气雾剂（经典烟草口味）不会导致支气管上皮培养物或肺泡三培养物中的细胞毒性，尽管其沉积的尼古丁浓度更高（分别为 3 倍和 4 倍）。 CS暴露导致支气管培养物中纤毛跳动的频率和活跃面积显着减少，而IQOS MESH气溶胶暴露则没有。整体 mRNA 表达和分泌蛋白谱显示，IQOS MESH 气溶胶暴露的影响明显低于 3R4F CS 暴露的影响。总体而言，我们的整个气溶胶暴露研究表明，即使在更大的尼古丁剂量下，相对于 CS，IQOS MESH 气溶胶对支气管和肺泡培养物的影响也明显降低。
京公网安备 11010802027423号