Novel device prototyping for endoscopic cell sheet transplantation using a three-dimensional printed simulator,Regenerative Therapy

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Novel device prototyping for endoscopic cell sheet transplantation using a three-dimensional printed simulator
Regenerative Therapy ( IF 3.4 ) Pub Date : 2020-11-17 , DOI: 10.1016/j.reth.2020.10.007
Hiroaki Osada ₁ , Wen-Jin Ho ₁ , Hideki Yamashita ₂ , Kazuhiro Yamazaki ₁ , Tadashi Ikeda ₁ , Kenji Minatoya ₁ , Hidetoshi Masumoto _{1,

3}

Affiliation

Introduction

Considering higher risks of candidates for cardiac regenerative therapy with compromised cardiac function, it is anticipated to develop less invasive surgical procedures. In the present study, we aimed to develop a prototype of totally endoscopic cell sheet delivery device and evaluate the surgical technique for epicardial cell sheet placement using three-dimensional (3D) printed simulators based on human computed tomography data.

Methods

We designed an endoscopic cell sheet delivery device with outer and inner frame with self-expandable applicator which can be opened in thoracic cavity. We launched spout line to provide liquids on the applicator surface and tension line to gently bend the applicator dorsally. We prepared human mesenchymal stem cell (MSC) sheets and compared wet/dry conditions of 3D printed heart/porcine heart and applicator to identify suitable conditions for cell sheet transplantation. Finally we validated the feasibility of endoscopic transplantation to anterior and lateral wall of left ventricle using 3D printed simulators.

Results

Moist condition of both 3D printed heart/porcine heart surface and applicator at transplantation yielded highest successful rate (100%, p = 0.0197). For both endoscopic transplantation sites, MSC sheets were successfully deployed. The procedure duration was 157 ± 23 s for anterior wall and 123 ± 13 s for the lateral wall in average, respectively.

Conclusions

We developed a novel prototype of endoscopic cell sheet delivery device for minimally-invasive cardiac regenerative therapy utilizing a 3D printed simulator. The commercialization of the prototype may provide a safe minimally-invasive method to deliver potential cardiac regenerative therapy in the future.

中文翻译：

使用三维打印模拟器进行内窥镜细胞片移植的新型设备原型

介绍

考虑到心脏功能受损的心脏再生治疗候选者的风险较高，预计将开发侵入性较小的外科手术。在本研究中，我们旨在开发一种全内窥镜细胞片输送装置的原型，并基于人体计算机断层扫描数据评估使用三维 (3D) 打印模拟器的心外膜细胞片放置手术技术。

方法

我们设计了一种带有外框和内框的内窥镜细胞片输送装置，该装置带有可在胸腔内打开的自膨胀涂药器。我们推出了喷嘴线以在涂抹器表面提供液体，并推出张力线以轻轻弯曲涂抹器背侧。我们准备了人类间充质干细胞 (MSC) 片材，并比较了 3D 打印心脏/猪心脏和涂药器的湿/干条件，以确定适合细胞片移植的条件。最后，我们使用 3D 打印模拟器验证了内窥镜移植到左心室前壁和侧壁的可行性。