In this study, we present the photosynthetic oxygen (O₂) supply to mammalian cells within a volumetric extracellular matrix-like construct, whereby a three-dimensional (3D)-bioprinted fugitive pattern encapsulating unicellular green algae, Chlamydomonas reinhardtii, served as a natural photosynthetic O₂ generator. The presence of bioprinted C. reinhardtii enhanced the viability and functionality of mammalian cells while reducing the hypoxic conditions within the tissues. We were able to subsequently endothelialize the hollow perfusable microchannels formed after enzymatic removal of the bioprinted C. reinhardtii-laden patterns from the matrices following the initial oxygenation period to obtain biologically relevant vascularized mammalian tissue constructs. The feasibility of co-culture of C. reinhardtii with human cells, the printability and the enzymatic degradability of the fugitive bioink, and the exploration of C. reinhardtii as a natural, eco-friendly, cost-effective, and sustainable source of O₂ would likely promote the development of engineered tissues, tissue models, and food for various applications.

在这项研究中，我们展示了在类似体积的细胞外基质结构中向哺乳动物细胞供应光合氧（O ₂ ），其中封装单细胞绿藻莱茵衣藻的三维（3D）生物打印逃逸模式作为天然的光合O ₂发生器。生物打印的存在C .莱茵哈特可以增强哺乳动物细胞的活力和功能，同时减少组织内的缺氧条件。随后，我们能够将生物打印的C酶去除后形成的中空可灌注微通道内皮化。在初始氧合期后，从基质中提取莱茵哈特氏菌负载模式，以获得生物学相关的血管化哺乳动物组织构建体。 C共培养的可行性。莱茵哈特菌与人体细胞的结合、逃逸生物墨水的可打印性和酶降解性，以及对莱茵衣藻的探索。莱茵哈特作为一种天然、环保、经济且可持续的 O ₂来源，可能会促进工程组织、组织模型和各种应用食品的开发。