Parkinson disease is the second most common neurodegenerative disease affecting elderly patients. It occurs due to the progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc). We continue our work in this model focusing on other brain areas affected with this disorder; cerebral cortex and cerebellum (areas other than substantia nigra) for better understanding the motor and behavior effect of the Parkinson disease as a forward steep for its treatment and medical control. This work aims to evaluate the therapeutic effect of stem cell-conditioned medium in the Parkinsonism model. In this study, Parkinsonism model was induced in rats by daily subcutaneous injection of 0.5 mg/Kg of rotenone for 28 days. Thirty rats were divided randomly into 3 groups; control, Parkinson, and conditioned medium (CM) treated groups. Cerebral Cortex and Cerebellum were obtained for histological, immunohistochemical and biochemical studies. In the Parkinsonism group, marked histological changes were observed. These findings were nearly ameliorated in CM treated group as confirmed by the biochemical, histological, and immunohistochemical (anti-alpha synculein, anti GFAP and anti nestin) studies. It could be concluded that CM had a good therapeutic effect on Parkinsonism induced damage in both the cerebral cortex and cerebellum.

中文翻译：

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骨髓源性干细胞条件培养基可改善鱼藤酮诱发帕金森病大鼠动物模型的脑和小脑组织学变化

帕金森病是影响老年患者的第二大最常见的神经退行性疾病。它的发生是由于黑质致密部 (SNpc) 中多巴胺能神经元的进行性退化。我们继续在这个模型中工作，重点关注受这种疾病影响的其他大脑区域；大脑皮层和小脑（黑质以外的区域）以更好地了解帕金森病的运动和行为影响，作为其治疗和医疗控制的前倾。这项工作旨在评估干细胞条件培养基在帕金森模型中的治疗效果。在这项研究中，通过每天皮下注射 0.5 mg/Kg 鱼藤酮 28 天在大鼠中诱导帕金森病模型。30只大鼠随机分为3组；对照、帕金森和条件培养基 (CM) 处理组。获得大脑皮层和小脑用于组织学、免疫组织化学和生化研究。在帕金森症组中，观察到显着的组织学变化。这些发现在 CM 治疗组中几乎得到改善，正如生化、组织学和免疫组织化学（抗 α 突触核蛋白、抗 GFAP 和抗巢蛋白）研究所证实的那样。可以得出结论，CM对帕金森病引起的大脑皮层和小脑损伤都有很好的治疗作用。抗 GFAP 和抗巢蛋白）研究。可以得出结论，CM对帕金森病引起的大脑皮层和小脑损伤都有很好的治疗作用。抗 GFAP 和抗巢蛋白）研究。可以得出结论，CM对帕金森病引起的大脑皮层和小脑损伤都有很好的治疗作用。