A label-free electrochemical method was developed for sensitive determination of ten-eleven translocation protein 1 (TET1) which can mediate the demethylation of DNA. This strategy is mainly based on MspI-mediated restriction endonuclease reaction. Current response difference of the biosensor before and after cleavage by MspI was dependent on the activity and concentration of TET1. With the aid of Au nanoparticles, this method shows a good linear range from 0.0042 μg μL-1 to 0.0210 μg μL -1 with a correlation coefficient of 0.9350 and a low limit of detection 0.00098 μg μL -1. Finally, this method was used to investigate the effects of n-oxalylglycine (NOG) and taxol on activity of TET1. The results indicated that NOG could inhibit TET1 activity but taxol could not. So this electrochemical biosensor could be applied to TET activity evaluation and inhibitor screening in field of biomedicine and clinical diagnosis.

中文翻译：

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用于灵敏测定 10-11 易位蛋白 1 的新型无标记电化学策略

开发了一种无标记电化学方法，用于灵敏测定可介导 DNA 去甲基化的 10-11 易位蛋白 1 (TET1)。该策略主要基于 MspI 介导的限制性内切核酸酶反应。MspI 切割前后生物传感器的电流响应差异取决于 TET1 的活性和浓度。借助Au纳米颗粒，该方法在0.0042 μg μL-1至0.0210 μg μL -1 之间显示出良好的线性范围，相关系数为0.9350，检测下限为0.00098 μg μL -1。最后，该方法用于研究正草酰甘氨酸 (NOG) 和紫杉​​醇对 TET1 活性的影响。结果表明NOG可以抑制TET1的活性，而紫杉醇则不能。