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Host reaction of tomato varieties and applicability of Sm -linked DNA markers to Stemphylium lycopersici
Molecular Breeding ( IF 2.6 ) Pub Date : 2020-11-18 , DOI: 10.1007/s11032-020-01188-8
Jiyeon Park , Seungahn Kwon , Girim Park , Siyoung Lee , Byoung Il Je , Younghoon Park

Gray leaf spot (GLS) disease, caused by the Stemphylium (Sm) fungus, affects a wide range of horticultural crops including tomato. In this study, the South Korean Sm isolate (PNU-SM01) was identified, and the applicability of Sm-resistance gene (Sm)-linked DNA markers in marker-assisted selection was validated. ITS/gpd sequencing and morphological observation identified the isolate as S. lycopersici. A GLS disease assay was conducted on 80 tomato varieties, including commercial F1 hybrids and elite inbred lines. The reference line ‘Motelle’ was highly resistant (R, DSI = 4.0), whereas the ‘Moneymaker’ line was susceptible (S) and showed severe disease symptoms (DSI = 79.3). Among 30 commercial F1 hybrids, five were scored as S by DSI; whereas the rest, including all 17 hybrids claimed as Sm-resistant by the supplier, were R or moderately resistant (MR). Among 48 inbred lines, four were scored as S and 44, including five Lycopersicon accessions reported as R, were R or MR. Three known Sm-linked DNA markers (CT55, D5, and Sm-InDel) were genotyped in all 80 cultivars; the highest match (92.5%) to the GLS disease assay was observed for Sm-InDel. We converted the recessive marker CT55 into a codominant marker (CT55-Co) and developed dCAPS (Sm-SNP) and KASP (Sm-KASP) markers from a previously identified Sm-linked SNP. Sm-SNP and Sm-KASP showed a similar level of phenotype to marker genotype association as Sm-InDel, whereas CT55-Co displayed a high level of mismatch. To screen for the source of Sm resistance, 138 tomato accessions were genotyped with Sm-InDel and Sm-SNP: 15 were homozygous for the resistance allele of both markers. In this study, resistance to the Korean isolate of S. lycopersici in commercial F1 hybrids and breeding sources was characterized to reveal which DNA markers can be effectively applied for marker-assisted selection of Sm-resistance.



中文翻译:

番茄品种的寄主反应及Sm连接的DNA标记在番茄中的应用

灰色叶斑病(GLS)病,引起的匍柄霉(SM)的真菌,影响很多园艺作物包括番茄的。在这项研究中,确定了韩国Sm分离株(PNU-SM01),并验证了Sm抗性基因(Sm)连锁的DNA标记在标记辅助选择中的适用性。ITS / gpd测序和形态学观察鉴定出该分离物为S. lycopersici。对80个番茄品种(包括商业F 1杂种和优良自交系)进行了GLS疾病分析。参考系“ Motelle”具有高度抗性(R,DSI = 4.0),而“ Moneymaker”系易感(S)并显示出严重的疾病症状(DSI = 79.3)。在30个商业F中1个杂种,5个被DSI评为S;而其余的,包括供应商声称具有Sm抗性的所有17个杂种,均为R或中度抗性(MR)。在48个自交系中,有4个被评为S,有44个,包括五个被报告为R的番茄属,分别为R或MR。在所有80个品种中对三种已知的Sm连接的DNA标记(CT55,D5和Sm-InDel)进行了基因分型。观察到Sm-InDel与GLS疾病分析的最高匹配率(92.5%)。我们将隐性标记物CT55转换为共性标记物(CT55-Co),并从先前鉴定的Sm中开发了dCAPS(Sm-SNP)和KASP(Sm-KASP)标记物连锁的SNP。Sm-SNP和Sm-KASP与Sm-InDel表现出相似的表型水平与标记基因型关联,而CT55-Co表现出高水平的错配。为了筛选Sm抗性的来源,用Sm-InDel和Sm-SNP对138个番茄种质进行了基因分型:两个标记的抗性等位基因中有15个纯合。在这项研究中,对商业F 1杂种和育种来源中的韩国番茄链球菌的抗性进行了表征,揭示了哪些DNA标记可以有效地用于标记辅助选择的Sm抗性。

更新日期:2020-11-18
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