In recent years, rice false smut (RFS) has grown from being a minor to a major disease affecting rice productivity. The genetic basis of the host’s defense against RFS is currently under-researched. In this study, a rice variety Nanjing11 with stable resistance to RFS from years under naturally infected field condition was identified. The genetic analysis showed that the RFS resistance of Nanjing11 was controlled by a single dominant gene, tentatively termed as false smut resistance 1 (FSR1), which was mapped to a 220-kb interval on the long arm of chromosome 1 by using an F₂ population bred from the cross Nanjing11 (RFS resistant) x CG3 (RFS susceptible). In this 220-kb region, DNMT2 (LOC_Os01g42630) was considered the most likely candidate gene for the RFS resistance of Nanjing11 on the basis of sequence variation and transcriptional responses to Ustilaginoidea virens infection. The result of this study lays the foundations for cloning and functional analysis of FSR1 and application of this gene.

近年来，稻曲病（RFS）已从影响水稻生产力的次要疾病变为主要疾病。目前，宿主对RFS防御的遗传基础尚未得到充分研究。在这项研究中，鉴定了在自然感染田间条件下多年稳定对RFS的水稻品种南京11。遗传分析表明，Nanjing11的RFS抗性受单个显性基因控制，暂定为假黑穗病抗性1（FSR1），通过使用F _2将其映射到1号染色体长臂上的220 kb区间。杂交后代Nanjing11（耐RFS）x CG3（易受RFS）繁殖。在此220 kb的区域中，DNMT2（LOC_Os01g42630）被认为是南京11 RFS抗性的最可能候选基因，基于序列变异和对Ustilaginoidea virens感染的转录反应。该研究结果为FSR1的克隆，功能分析和该基因的应用奠定了基础。