Purpose

Sediment oxygen demand (SOD) measurement currently requires a long preparation time and bulky experimental equipment, which represent major obstacles to conducting SOD measurements. We developed a new SOD measurement method that can be easily conducted in a shorter time than the existing laboratory method.

Materials and methods

The undisturbed sediment core was transferred into the custom-made small glass vial (inner diameter 12.8 mm, length 125 mm). The SOD sample was incubated in the dark with or without stirring the overlying water inside the vial. The change in DO concentration was measured using a non-contact fluorescent oxygen sensor, and then the SOD was calculated. For comparison and verification of the new method, SOD was also measured using a large undisturbed sediment core (inner diameter 11 cm, length 50 cm).

Results and discussion

The SOD measurements using the new method were positively correlated with the SOD measurements using the large undisturbed sediment core, suggesting that the new method is a feasible alternative to the conventional method. In the new method, many SOD measurements can be taken from a single sediment core sample. This makes it possible to reduce the uncertainty associated with the highly heterogeneous structure of the sediments, and thus the new method has great advantages in obtaining reliable SOD data.

Conclusions

A novel high-throughput method for SOD was developed that shortens sample preparation time and improves convenience of measurement. The new method is expected to contribute substantially to the accumulation of SOD monitoring data and to make great progress in elucidating the dynamics of dissolved oxygen in lakes.

中文翻译：

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开发一种新的高通量小型方法来测量湖泊中的沉积物需氧量

目的

目前，沉积物需氧量（SOD）的测量需要较长的准备时间和庞大的实验设备，这是进行SOD测量的主要障碍。我们开发了一种新的SOD测量方法，与现有的实验室方法相比，它可以在更短的时间内轻松完成。

材料和方法

将原状的沉淀物芯转移到定制的小玻璃小瓶中（内径12.8毫米，长125毫米）。将SOD样品在有或没有搅拌小瓶内上覆水的黑暗环境中孵育。使用非接触式荧光氧气传感器测量DO浓度的变化，然后计算SOD。为了比较和验证新方法，还使用了一个大的未扰动沉积物芯（内径11厘米，长度50厘米）测量了SOD。

结果和讨论

使用新方法的SOD测量值与使用大面积原状沉积物芯的SOD测量值呈正相关，这表明该新方法是常规方法的可行替代方案。在新方法中，可以从单个沉积物岩心样品中进行许多SOD测量。这使得减少与沉积物高度非均质结构有关的不确定性成为可能，因此，该新方法在获得可靠的SOD数据方面具有很大的优势。

结论

开发了一种新颖的高通量SOD方法，可缩短样品制备时间并提高测量便利性。新方法有望大大促进SOD监测数据的积累，并在阐明湖泊中溶解氧的动态方面取得重大进展。